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EC number: 940-543-9
CAS number: 354-15-4
of the general body condition
showed that in the first 7-10 days of the experiment the animals that
were exposed to HCFC 122a at a concentration of 1000 mg/m3developed
apathy and lethargy. However, the observed changes disappeared by the
end of the first month of the experiment.
to HCFC 122a at concentrations of 10 and 100 mg/m3did not
cause changes in appearance and behaviour of the experimental animals.
of the dynamics of body weight in rats showed that HCFC 122a in the
highest concentration caused significant reduction in body weight gain
starting from the third month of the experiment. However, during the
recovery period, the difference in body weight in this group of
animals levelled up.
to HCFC 122a at concentrations of 10 and 100 mg/m3caused no
significant changes in weight of the experimental animals as compared
to the control group.
on the morphological composition of the blood.
of the peripheral blood revealed that HCFC 122a at a concentration of
1000 mg/m3caused a statistically significant decrease in
the erythrocyte count starting from the third month of the experiment
and an increase in the leukocyte count in peripheral blood.
recovery period, the leukocyte count returned to the level of the
control group, but the erythrocyte count remained significantly
reduced. No change in hemoglobin count was observed during the entire
to the refrigerant at a concentration of 100 mg/m3only
increased the leukocyte count in blood at the end of the experiment.
concentration of 10 mg/m3, HCFC 122a did not cause
morphological changes in blood throughout the entire chronic
count in peripheral blood
count in blood
content in blood
on the nervous system.
functional state of the nervous system of experimental animals in the
chronic experiment was assessed using the following indicators:
summation-threshold value, orientation-exploratory activity, and the
Study of the
functional state of the nervous system using the summation-threshold
value showed that the most pronounced changes in this indicator were
observed in animals exposed to HCFC 122a at a concentration of 1000 mg/m3,
which were noted already starting from the second month of the
experiment. It should be noted that the detected change did not get to
normal during the recovery period.
concentration of 100 mg/m3, the refrigerant caused changes in
summation-threshold value only in the first month of experience, but
later on there was no difference from the control group.
HCFC 122a at
a concentration of 10 mg/m3caused only a tendency to changes
in the summation-threshold value at the end of the experiment.
the changes in the summation-threshold value, a significant reduction in
exploratory activity in laboratory animals was also observed.
significant changes in this indicator took place upon exposure to HCFC
122a at a concentration of 1000 mg/m3. Identified changes
were persistent and no reversibility of the effects was observed by the
end of the recovery period.
concentration of 100 mg/m3, HCFC 122a caused changes in the
exploratory reaction of rats at the end of the 4th month of the
experiment. No changes in this indicator were observed upon exposure to
the substance at a concentration of 10 mg/m3.
The study of
the “split” value characterizing the state of the neuromuscular system
revealed only a trend towards its increase in the group of animals
exposed to the substance at a concentration of 1000 mg/m3.
studies to assess the functional state of the nervous system in case of
chronic poisoning with HCFC 122a revealed that the studied compound has
a pronounced effect on the nervous system, reducing the activity of
nerve cells and lowering the lability of nervous processes.
of changes in summation-threshold value (STV)
on the functional state of the liver.
function was assessed using tests that were sensitive while determining
the threshold acute effect. Study on the activity of sorbitol
dehydrogenase (SDH), lactate dehydrogenase-5 (5-LDH) specific to the
liver tissue and transaminases were conducted.
studies showed that HCFC 122a at a concentration of 1000 mg/m3can
cause increased activity of SDH from the first month of the experiment,
and the activity of this enzyme in blood serum remained high till the
end of the experiment.
in this indicator were observed upon exposure to the substance at a
concentrations of 10 and 100 mg/m3.
The study of
5-LDH isoenzyme allowed to establish its significant increase in blood
serum throughout the experiment in the group of animals exposed to the
substance at a concentration of 1000 mg/m3and a tendency to
increased activity of the enzyme at the end of experiment.
HCFC 122a at the lower tested concentration did not change this
of transaminases activity in the blood serum upon exposure to the
refrigerant allowed to observe significant increase in activity of this
enzyme at the end of the first month of the experiment at a
concentration of 1000 mg/m3.
of the study of SH groups content in the peripheral blood of
experimental animals showed that at the lowest tested concentrations (10
and 100 mg/m3) HCFC 122a caused no significant alterations of
this indicator. Exposure to a concentration of 1000 mg/m3caused
significant decrease in SH groups in the blood at the end of the 4th
month of the experiment; however, one month after cessation of
administartion, the content of SH groups in the blood of animals of this
group did not differ from controls.
of sorbitol hydrogenase in the blood serum
group content in blood (general)
group content in blood (non-protein)
The study of
the histamine content in the blood of experimental animals allowed to
establish a significant decrease of this index when exposed to HCFC 122a
at a concentration of 1000 mg/m3. At a concentration of 100
mg/m3, the substance caused just a tendency to reduction of
the histamine content in the blood, and at a concentration of 10 mg/m3,
this indicator did not significantly different from the control group.
content in blood
subchronic toxicological study was conducted by the Ministry of Health
of the Russian Federation in order to establish the threshold of chronic
action and validate the maximum allowable concentration of HCFC 122a in
the air of the working area.
was performed on 3 groups of 20 male rats each, at the concentration of
10, 100, and 1000 mg/m3. A control group was included in the experiment.
plan was defined taking into account the existing data on the biological
action of HCFC 122a and structurally related compounds and the physical
and chemical properties of the substance.
Throughout the 4 -month administration, behaviour of animals, their
appearance and weight were observed. Since similar compounds are known
to have effects on the nervous system, test of neuro-muscular
activity, motor activity, orientation response and “emotionality” were
Analysis on the activity of sorbitol dehydrogenase, lactate
dehydrogenase-5 specific to the liver tissue and transaminases were
conducted in order assess potential functional changes in the
Furthermore, since published data showed that similar compounds
can affect the morphological composition of the blood,analysis of
peripheral blood components morphology and of the immunological
reactivity was performed. Observations of the functional state of
the experimental animals were carried out on a monthly basis.The
first examination was carried out on Day 10. In
addition, in order to determine the stability of the identified changes,
the state of the animals was assessed one month after the termination of
administration. At the end of the experiment, the
animals were sacrificed, followed by determination of weight factors of
the internal organs and post-mortem examinations.
Statistical data processing was carried out using conventional methods
with the calculation of mean values, their errors and Student’s t-test.
showed that the concentration of HCFC 122a of 1000 mg/m3can
be described acting in the chronic experiment based on indicator
reflecting the state of the nervous system, peripheral blood, and liver.
Tests to assess the functional state of the nervous system in case of
chronic exposure to HCFC 122a revealed that the studied compound has a
pronounced effect on the nervous system at 1000 mg/m3,
reducing the activity of nerve cells and lowering the liability of
the above described results a NOAEC of 100 mg/m3 can be extrapolated.
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