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EC number: 940-543-9
CAS number: 354-15-4
of the study of mutagenic activity of R122 on the test strains
Concentration % by volume or mcg/dish
Average number of his. revertants per dish in options
e. = toxic effect
from the dose of 10 mcg/dish, the substance inhibits growth of bacteria.
objective of the reported study was the assessment both of the
reprotoxicity potential and of the genotoxicity potential of HCFC 122a.
To the latter scope, an in vivo micronucleus test and an Ames test were
conducted in accordance with the “Guidelines for study of mutagenic
activity of drugs” approved by the Ministry of Health of theon January
15, 1988, using strains of Salmonella typhimurium TA 98, TA 100, TA 97.
of mutagenic effect in the preparation was determined by induction of
back mutations from histidine auxotrophy to prototrophy.
was tested following the plate incorporation method at the
concentrations of 0.01, 0.1, 1, 10, 100 mcg/plate. The analysis of the
results was performed after 48-72 hours of incubation.
the experiment included options with FMAM (S9 Full microsomal activation
mixture) and PMAM (S9 Partial microsomal activation mixture).
PMAM can demonstrate action of direct mutagens, i.e. drugs that exhibit
mutagenic effect due to the activity of the original structure of the
substance. The action of the promutagens, i.e. compounds, the effect of
which is associated with the formation of mutagenic metabolites, may be
taken into account when comparing the results of analysis of test
substances in options FMAM and PMAM.
control option, FMAM and PMAM were introduced into the upper layer of a
semi-solid agar together with the bacterial suspension and the
corresponding volumes of solvent. The experiment was accompanied by
were used in each control and experimental option. The experiment was
were registered in case of mutagenic effect in all positive control
performed using Dunnett’s multiple comparison method.
in S. typhimurium test strains were observed. Positive controls
effectively induced mutations in the respective S. typhimurium test
strains with and without metabolic activation. Starting from the dose of
10 mcg/dish, the preparation inhibits growth of bacteria.
conclusion, under test conditions, HCFC 122a did no have mutagenic
activity when tested on standard strains TA 100, TA 98, TA 97.
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