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EC number: 940-543-9
CAS number: 354-15-4
Summary of Results
Number of animals
Polychromatocytes with micronuclei Mav. ± M
The objective of the study was the
assessment both of the reprotoxicity potential and of the genotoxicity
potential of HCFC 122a. To this scope, an in vivo micronucleus
test and an Ames test were performed.
The micronucleus test was conducted on white
mice weighing 18-20 g.
HCFC 122a was administered to mice twice
with the intervals of 24 hours, at doses of 1/2 and 1/10 of the LD50 =
7800 mg/Kg. The animals were sacrificed in 24 hours after the last
Femoral bones were taken and the bone marrow
was placed into a drop of group IV human serum inactivated at 56 °C for
2 hours and took a smear. After drying of preparations in air, the smear
was consequently stained with Main-Grunwald and Giemsa stains.
Analysis of bone marrow smear was performed
using the immersion microscope objective Jenaval (magnification
H/100x12.5¥0.17-A). Preparations with well expanded
erythrocytes, the surface of which did not have protuberances and folds
were considered suitable for the analysis. 1000 polychromatocytes were
counted per each animal.
HCFC 122a mutagenicity was assessed by the
presence or absence of difference in the number of micronuclei between
the experimental and control animals. Data are shown as a number of
polychromatocytes containing micronuclei in percent. For the statistical
evaluation, the original data for each animal were standardized
according to the formula:
= arc sin [(r+0.375)/(n+0.75)] 1/2
– number polychromatophils with micronuclei
– the total number of polychromatocytes,
used tst– Student’s t test.
obtained indicate that the incidence of micronuclei in the bone marrow
cells of experimental mice for the studied doses was not statistically
different from the control values.
Thus, the studied
dosage range of HCFC 122a upon oral administration to mice in
micronucleus test did not have mutagenic activity.
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