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EC number: 202-160-9
CAS number: 92-50-2
Table 1: Induction of Luciferase Activity – Overall Induction
= significant induction according to Student’s t-test, p<0.05
Table 2: Acceptance Criteria
CV Solvent Control
No. of positive control concentration steps with significant luciferase activity induction >1.5
± 2 x SD of historical mean
Induction PC at 64 µM
2.00 < x < 8.00
The in vitro KeratinoSens™
assay enables detection of the sensitising potential of a test item by
addressing the second molecular key event of the adverse outcome pathway
(AOP), namely activation of keratinocytes, by quantifying the luciferase
activity in the transgenic cell line KeratinoSens™. The luciferase
activity, assessed by luminescence measurement, compared to the
respective solvent controls is used to support discrimination between
skin sensitisers and non-sensitisers.
In the present
study 2-(N-ETHYLANILINO)ETHANOL was dissolved in DMSO. Based on a
molecular weight of 165.24 g/mol a stock solution of 200 mM was
on the stock solution a set of twelve master solutions in 100% solvent
was prepared by serial dilution using a constant dilution factor of 1:2.
These master solutions were diluted 1:100 in cell culture medium. The
following concentration range was tested in the assay: 2000,
1000, 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 3.91, 1.95, 0.98 µM.
incubated with the test item for 48 h at 37°C. After exposure cells were
lysed and luciferase activity was assessed by luminescence measurement.
In two separate experiments, no
significant luciferase induction > 1.5 was found in the tested
concentration range. Therefore, no EC1.5value could be
No dose response for luciferase
activity induction was observed for each individual run as well as for
an overall luciferase activity induction. Under
the condition of this study the test item is therefore considered as non
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