Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-160-9
CAS number: 92-50-2
The mixture of 25 mg test item per 1 mL MTT medium showed no reduction
of MTT compared to the solvent. The mixture did not turn blue/purple.
Therefore, NSMTT equalled 0%. The mixture of 25 mg of the test item per
300 µl aqua dest. and per 300 µL isopropanol showed no colouring
detectable by unaided eye-assessment. Therefore, NSC equalled 0%.
Table 1: Results of the main experiment:
Mean OD570of the Duplicates (Blank Corrected)
Total Mean OD570of 3 Replicate Tissues (Blank Corrected)
Relative Tissue Viability [%]
Mean Relative Tissue Viability [%]
SD Tissue Viability [%]***
CV [% Viabilities]
* Blank-corrected mean OD570 nmof the negative control
corresponds to 100% absolute tissue viability.
** Mean relative tissue viability of the three positive
control tissues is£ 20%.
*** Standard deviation (SD) obtained from the three
concurrently tested tissues is≤ 18%
Table 2: Quality criteria
Mean Absolute OD570 nmNK
0.8 ≤ NK ≤2.8
Mean Relative Viability [%] PC
0.1 – 1.9
In the present study the skin corrosivity potential of
2-(N-ethylanilino)ethanol was analysed. Since corrosive chemicals are
cytotoxic after a short time exposure to the stratum corneum of the
epidermis the cytotoxic effects of the test item on EpiDermÔ, a
reconstituted three-dimensional human epidermis model, were determined.
Hereby, the test item was applied topically. Cytotoxicity is expressed
as the reduction of mitochondrial dehydrogenase activity measured by
formazan production from MTT after a 3 min and 60 min exposure period
and compared to those of the concurrent negative controls.
The mixture of 50 µL test item per 1 mL MTT medium showed no
reduction of MTT as compared to the solvent. The mixture did not turn
blue/purple.Therefore, NSMTT equaled 0%.
The mixture of 50 µL test item per 300 µL Aqua dest.and per 90 µL
isopropanolshowed no colouring as compared to the solvent.Therefore NSC
In the first experiment, the test item showed no corrosive
effects. The mean relative tissue viability (% negative control)
was³ 50% (78.7%) after 3 min treatment and³ 15% (15.5%) after 60 min
treatment. However, the mean viability (15.5%) after 60 min treatment
was near to the threshold of 15% and also the two tissue replicates
showed inconclusive results. One tissue replicate was above (15.9%) and
one below (14.4%) the 15% threshold after 60 min treatment. Thus, as
recommended by the OECD guideline, a second experiment was performed.
In the second experiment, the test item showed corrosive effects.
The mean relative tissue viability (% negative control) was reduced
below 15% (7.4%) after 60 min treatment but not below 50% (104.9%) after
3 min treatment. Both tissue replicates were below (7.3% and 7.5%) the
threshold of 15% after 60 min treatment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Sellel veebilehel kasutatakse küpsiseid, et tagada lehe parim kasutus.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again