Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 September 2015 to 18 April 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Version / remarks:
2006
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
EC No. 761/2009
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Physical state/Appearance: Brown liquid
Batch: 15B26
Purity: 81.7%
Expiry date: 1 August 2016
Storage conditions: Room temperature in the dark under nitrogen
Analytical monitoring:
yes
Vehicle:
yes
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Test type:
other: Constat shaking at approx. 150 rpm for 72h
Water media type:
other: culture medium
Limit test:
no
Total exposure duration:
72 h
Remarks on exposure duration:
72 h exposure under continuous illumination and shaking at 150 rpm at 24 deg C
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
130 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other:
Remarks:
Value determined from the equation of the fitted line as no concentration tested resulted in 50% inhibition of growth rate.
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
34 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Remarks:
95% confidence LImites 28 - 40 mg/L
Validity criteria fulfilled:
yes
Remarks:
Validation criteria: Cell concentration in controlmust increase by a factor of at least 16 . Mean C of V in the daily growth rates in control must not exceed 35%. Ct of V of average specific growth rate in replicate control cultures must not exceed 7%.
Conclusions:
Growth rate:
- EC50(mg/L): 130 (Values determined from the equation for the fitted line as no concentration tested resulted in 50% inhibition of the growth rate)
- 95% confidence limits (mg/L): It was not possible to calculate 95% confidence limits for the ErC50 value as the data generated did not fit the models available for the calculation of the confidence limit.
- NOEC (mg/L): 10
- LOEC (mg/L): 32
Yield:
- EC50(mg/L): 34
- 95% confidence limits (mg/L): 28-41
- NOEC (mg/L): 10
- LOEC (mg/L): 32

Description of key information

Response variable            EC50(mg/L)              95% confidence limits (mg/L)              NOEC (mg/L)              LOEC (mg/L)

Growth rate                       130*                          **                                                      10                           32

Yield                                   34                            28 -41                                                 10                              32

* Values determined from the equation for the fitted line as no concentration tested resulted in 50% inhibition of the growth rate

** It was not possible to calculate 95% confidence limits for the ErC50 value as the data generated did not fit the models available for the calculation of the confidence limit.

Key value for chemical safety assessment

EC50 for freshwater algae:
130 mg/L

Additional information

Categories Display