2-Propanol, 1-butoxy-3-(2-propen-1-yloxy)-

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 September 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Version / remarks:

2010

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Physical state/Appearance: brown liquid
Batch: 15B26
Purity: 81.7%
Expiry Date: 01 August 2016
Storage Conditions: room temperature in the dark under nitrogen

Analytical monitoring:

not required

Vehicle:

yes

Remarks:

Water

Test organisms (species):

activated sludge of a predominantly domestic sewage

Test type:

other: bubble aeration with clean compressed air 0.5-1.0 L/min.

Water media type:

other: deionized reverse osmosis water

Remarks:

<1 mg/mL dissolved organic carbon

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 deg C

pH:

7.1

Dissolved oxygen:

5.7-8.3 mg O2/L

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol included in range-finding test at 3.2, 10 and 32 Mg/L to confirm suitabiiity of inoculum

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

100 - 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Validity criteria fulfilled:

yes

Conclusions:

The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 value of greater than 1000 mg/L.

Description of key information

Respiration of activated sewage sludge micro-organisms:

3-Hour EC₅₀ : >1000 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L