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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 September 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
Version / remarks:
2010
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Physical state/Appearance: brown liquid
Batch: 15B26
Purity: 81.7%
Expiry Date: 01 August 2016
Storage Conditions: room temperature in the dark under nitrogen


Analytical monitoring:
not required
Vehicle:
yes
Remarks:
Water
Test organisms (species):
activated sludge of a predominantly domestic sewage
Test type:
other: bubble aeration with clean compressed air 0.5-1.0 L/min.
Water media type:
other: deionized reverse osmosis water
Remarks:
<1 mg/mL dissolved organic carbon
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20 deg C
pH:
7.1
Dissolved oxygen:
5.7-8.3 mg O2/L
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol included in range-finding test at 3.2, 10 and 32 Mg/L to confirm suitabiiity of inoculum
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
100 - 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Validity criteria fulfilled:
yes
Conclusions:
The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 value of greater than 1000 mg/L.

Description of key information

Respiration of activated sewage sludge micro-organisms:

3-Hour EC50 : >1000 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

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