Registration Dossier
Registration Dossier
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EC number: 202-716-0 | CAS number: 98-95-3
Exposure related observations in humans: other data
Administrative data
- Endpoint:
- exposure-related observations in humans: other data
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- Percutaneous absorption of nitroaromatic compounds: in vivo and in vitro studies in the human and monkey.
- Author:
- Bronaugh, R.L. and Maibach, H.I.
- Year:
- 1 985
- Bibliographic source:
- Journal of Investigative Dermatology 84, 180-183
Materials and methods
- Type of study / information:
- Absorption through human skin
- Endpoint addressed:
- dermal absorption
- Principles of method if other than guideline:
- Absorption through human skin was measured by using excised skin in diffusion cells. The relative volatility of each compound was quantitated in seperate experiments by measuring the loss of radioactivity from waxed paper or epidermal-discs.
- GLP compliance:
- no
Test material
- Reference substance name:
- Nitrobenzene
- EC Number:
- 202-716-0
- EC Name:
- Nitrobenzene
- Cas Number:
- 98-95-3
- Molecular formula:
- C6H5NO2
- IUPAC Name:
- nitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): nitrobenzene
- Analytical purity: no data
- Radiochemical purity (if radiolabelling): > 97%
- Specific activity (if radiolabelling): 6.6 mCi/mmol
- Locations of the label (if radiolabelling): 14C-labelling
Constituent 1
Method
- Details on study design:
- The absorption through excised human abdomonal skin was measured by in vitro diffusion cell techniques (static or flow-through). Dermatome sections of human skin were utilized in the permeability studies. Acetone was used as vehicle; test substance concentration was 4 µg/cm2. The site of application was washed with soap and water at 24 h in all experiments.
- Exposure assessment:
- measured
Results and discussion
- Results:
- In experiments using non-occluded skin, 7.8 ± 1.2% of the applied dose of nitrobenzene was absorbed percutaneously, whereas under conditions, where evapouration was prevented, percutaneous absorption raised up to 41.1 ± 2.0%. The evapouration of nitrobenzene from human epidermal discs at the site of application were 75.8, 30.4 and 21.9% of the applied dose after 1 min, 3 h and 24h.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
