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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable. Guideline study, well documented and with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1988
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: "Proposed Preliminary Testing Method: prolonged toxicity test on Daphnia magna", according to the Federal Environmental Agency .
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrobenzene
EC Number:
202-716-0
EC Name:
Nitrobenzene
Cas Number:
98-95-3
Molecular formula:
C6H5NO2
IUPAC Name:
nitrobenzene
Details on test material:
- Analytical purity: 99 %

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Sampling method: GC or HPLC, combined with FID ,ECD , FPD or MS

Test solutions

Vehicle:
not specified
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dilution series of the stock solution

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain/clone: IRCHA
- Source: Federal Envrionmental Agency
- Feeding during test
- Food type: Algae and TetraMin



Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

pH:
7.45 - 7.80
Dissolved oxygen:
Saturation of oxygen: about 77 - 90 %
Nominal and measured concentrations:
Nominal test concentrations: 1.56, 3.13, 6.25, 12.5, 25, 50, 100 and 200 mg/L

Details on test conditions:
TEST SYSTEM
- Test vessels filled with 250 ml test substance
- Type (delete if not applicable): open
- Renewal rate of test solution (frequency/flow rate): every 3 days
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: artificial freshwater (according to DIN)

OTHER TEST CONDITIONS
- Photoperiod: 9:15 (light:dark)

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
2.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Analytical determined value
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

Any other information on results incl. tables

Recovery rates: 
12.5 mg/L (nominal): 21 -29 % recovery
25.0 mg/L (nominal): 14 -22 % recovery 
50.0 mg/L (nominal): 10 -26 % recovery

Applicant's summary and conclusion