Nitrobenzene

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable. Guideline study, well documented and with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1988

Reference 2

Reference Type:

secondary source

Title:

European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3

Author:

European Chemicals Bureau

Year:

2007

Bibliographic source:

3rd Priority List, Volume: 77

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: "Proposed Preliminary Testing Method: prolonged toxicity test on Daphnia magna", according to the Federal Environmental Agency .

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Nitrobenzene

EC Number:

202-716-0

EC Name:

Nitrobenzene

Cas Number:

98-95-3

Molecular formula:

C6H5NO2

IUPAC Name:

nitrobenzene

Details on test material:

- Analytical purity: 99 %

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: GC or HPLC, combined with FID ,ECD , FPD or MS

Test solutions

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dilution series of the stock solution

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: IRCHA
- Source: Federal Envrionmental Agency
- Feeding during test
- Food type: Algae and TetraMin

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

pH:

7.45 - 7.80

Dissolved oxygen:

Saturation of oxygen: about 77 - 90 %

Nominal and measured concentrations:

Nominal test concentrations: 1.56, 3.13, 6.25, 12.5, 25, 50, 100 and 200 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessels filled with 250 ml test substance
- Type (delete if not applicable): open
- Renewal rate of test solution (frequency/flow rate): every 3 days
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: artificial freshwater (according to DIN)

OTHER TEST CONDITIONS
- Photoperiod: 9:15 (light:dark)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

2.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: Analytical determined value

Effect concentrations 2

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Any other information on results incl. tables

Recovery rates: 
12.5 mg/L (nominal): 21 -29 % recovery
25.0 mg/L (nominal): 14 -22 % recovery 
50.0 mg/L (nominal): 10 -26 % recovery