Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-292-6
CAS number: 4292-10-8
Preliminary study:Mortality:1000 mg/kg bw: 4/4 animals died within 30 hours after first administration 2000 mg/kg bw: 4/4 animals died within 4 hours after first administration
In a OF1
(I.O.P.S. Caw) mouse bone marrow micronucleus assay, performed as
described by W. Schmid - The Micronucleus test, Mutation Research, 31,
9-15 (1975), 5 male and 5 female animals were treated i. p. with Coco
AAPB (30 % a.i). The test method by W. Schmidt is
almost equivalent to the procedure described by OECD guideline 474. In
a preliminary study the test animals were administered twice (in a 24
hours interval) each 100, 200, 500, 1000 and 2000 mg/kg bw/day by
intraperitoneal injection. Clinical signs and mortality were observed up
to 30 hours after the first administration. Clinical signs like
piloerection and ptosis were seen at doses of >/= 100 mg/kg bw/day. At
doses >/= 1000 mg/kg bw/day the mice died within 30 and 4 hours after
the first administration. The tolerated doses were in the range of 100
to 500 mg/kg bw/day. Therefore, the dose of 200 mg/kg bw/day was
selected as the high dose and 20 mg/kg bw/day (10 % of the high dose) as
the low dose. As the test substance was applied twice with a 24 h
interval (although only one timepoint was chosen for sacrifice), the
result of the sacrifice 6h later may be regarded as a result of a 30h
and a 6h treatment. The dose level of 200 mg/kg bw/day (corresponding to
60 mg active substance/kg bw/day) is considered to be sufficiently high
based on the effects found in the preliminary study and due to the
highly irritating properties of the compound.
The mean number of micronucleated
erythrocytes/1000 polychromatic erythrocytes in males and female mice at
20 and 200 mg/kg bw/day were unaffected compared to the negative
controls. The administration of 100 mg cyclophosphamide/kg bw serving as
the positive control led to clearly elevated numbers of micronucleated
It can be concluded, that Coco AAPB (30
% a.i) induced no clastogenic effect in this in vivo cytogenicity
study on mice at dose levels of 20 and 200 mg/kg bw/day.
is classified as acceptable. This study satisfies the requirement for
Test Guideline OECD 474 for in vivo cytogenetic mutagenicity
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again