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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-12-12
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non-GLP Guideline study, limited reporting

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
6-[3-(C12-C18)-alkenyl(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid
EC Number:
701-162-1
Molecular formula:
C22H37NO4 - C28H49NO4
IUPAC Name:
6-[3-(C12-C18)-alkenyl(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid
Details on test material:
Lot No. 3/90 (24.04.1990)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
500-1000 mg/L

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Kommunale Abwasserreinigungsanlage Frankfurt-Sindlingen, non adapted sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
none

Test conditions

Hardness:
n.a.
Test temperature:
20 +/- 2 deg C
pH:
7.2 +/- 0.2
Dissolved oxygen:
saturated
Reference substance (positive control):
yes
Remarks:
3,5 Dichlorophenol 5-20-30 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The registration substance did not inhibit the respiration rate of microorganisms in activated sludge at the highest tested concentration of 1000 mg/L in an OECD 209 test. The NOEC (3h) is 1000 mg/L.