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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-07-23 - 1991-08-22
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study, some deficiencies (no analytical monitoring)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
6-[3-(C12-C18)-alkenyl(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid
EC Number:
701-162-1
Molecular formula:
C22H37NO4 - C28H49NO4
IUPAC Name:
6-[3-(C12-C18)-alkenyl(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid
Details on test material:
liquid
Lot No. 3/90 (24.04.1990)
insoluble in water

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Test concentrations: 10-18-32-58-100-180 mg/L

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Supplier: Zoo-Stumpe, D-3200 Hildesheim
Length 3.0-3.2 cm
Weight: 0.33 g/fish
Feed: TeraMin, Fa. Tetra

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Test temperature:
23 +/- 2 deg C
pH:
7.86 (0h) - 8.16 (96h)
Dissolved oxygen:
Degree of oxygen saturation: 96-99%

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In a dose response OECD 203 acute fish toxicity test with the registration substance a LC50 (96h) of 100 mg/L was derived.