Registration Dossier
Registration Dossier
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EC number: 250-439-9 | CAS number: 31027-31-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: cited in BG 249 (CIT study)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- p-isopropylphenyl isocyanate
- EC Number:
- 250-439-9
- EC Name:
- p-isopropylphenyl isocyanate
- Cas Number:
- 31027-31-3
- Molecular formula:
- C10H11NO
- IUPAC Name:
- 1-isocyanato-4-(propan-2-yl)benzene
- Details on test material:
- no data
Constituent 1
Method
- Target gene:
- no data
Species / strain
- Species / strain / cell type:
- other: TA 1535, TA 1537, TA1538, TA98, TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- up to 5000 µg/plate
- Vehicle / solvent:
- Dimethylsulfoxide (DMSO)
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium: TA 98, TA 100, TA 1535, TA 1537, Ta 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 3000 µg/plate precipitation, 5000 µg/plate bacteriotoxic (TA 98 and TA 100)
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Neither with nor without S9-mix p-isopropylphenyl isocyanate showed mutagenic properties up to a concentration of 1500 µg/plate. With a concentration of 3000 µg/plate percipitation occurs and with 5000 µg/plate (but not with 10 to 1000 µg/plate) in a concentration range finding study on TA 98 and TA 100 a bacteriotoxic effect was seen.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
method: Ames test according OECD 471
result: p-isopropylphenyl isocyanate is not mutagenic
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