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The acute toxicity of 2 -Propanol, 1,1'-[[3 -[(3 -aminopropyl)amino]propyl]imino]bis-, N-tallow alkyl derivatives (Jenkins, 2011)

was evaluated with Oncorhynchus mykiss in a study performed in accordance with OECD testing guideline 203 and GLP. The 96-hr LC50 value for rainbow trout exposed to this substance was 78.9 µg/L.

In Jenkins (2011), the effect of 2-Propanol, 1,1'-[[3-3[(3-aminopropyl)amino]propyl]imino]bis-, N-tallow alkyl derivatives on the reproduction of Daphnia magna was determined in accordance with OECD testing guideline 211 and GLP. There was no adverse effect on reproduction at the concentrations where the parental animals survived, giving a NOEC of 32 µg/L and a LOEC >32 µg/L. Therefore, EC50 could not be determined.

The determination of the growth inhibition of the fresh algae Pseudokirchneriella subcapitata exposed to

2 -Propanol, 1,1'-[[3 -[(3 -aminopropyl)amino]propyl]imino]bis-, N-tallow alkyl derivatives for

a duration of 72h was performed following the method OECD 201 and according to GLP (Jenkins 2011). The 96 -hr EC50 for algae was 28.3 µg/L and the 96 -hr NOEC was 9.77 µg/L.