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EC number: 261-332-1
CAS number: 58567-11-6
The following results were obtained for (ethoxymethoxy)cyclododecane
using acetone:olive oil (4:1 v/v) as vehicle:
Test item concentration % (w/v)
Number of lymph nodes isolated
dpm / lymph nodea)
BG = Background (1 mL 5 % trichloroacetic acid) in duplicates
SI = Stimulation Index (Ratio = dpm/lymph node test item treated
group / dpm/lymph node Group 1)
a) = Since the lymph nodes of the animals of a dose group were
pooled, dmp/lymph node was determined by dividing the measured value by
the number of lymph nodes pooled.
Calculation of the EC3 Value
Test item concentration
EC3 = (a-c) [(3-d)/(b-d)] + c = 25.1 %
EC3 = Estimated concentration for a SI of 3
a, b, c, d = Co-ordinates of the two pairs of data lying
immediately above and below the SI value of 3 on the LLNA dose response
In order to study the possible contact allergenic potential of the
substance, three groups of four female mice each were treated daily with
the test item at concentrations of 10%, 20% and 40% (w/v) in
acetone:olive oil (4:1 v/v) by topical application to the dorsum of each
ear lobe for three consecutive days. A control group of four female mice
was treated with the vehicle acetone:olive oil (4:1 v/v) only.
Five days after the first topical application the mice were injected
intravenously into a tail vein with radio-labelled thymidine (3H-methyl
thymidine, 3HTdR). Approximately five hours after intravenous injection,
the mice were sacrificed, the draining auricular lymph nodes were
excised and pooled per group. Single cell suspensions of lymph node
cells were prepared from pooled lymph nodes which were subsequently
washed and incubated with trichloroacetic acid overnight. The
proliferative capacity of pooled lymph node cells was determined by the
incorporation of 3HTdR measured in a β-scintillation counter.
All treated animals survived the scheduled study period. Neither
clinical signs on the ears of the animals nor systemic findings were
observed during the study period.
The results obtained [Stimulation Index (S.I.)] are reported in the
following table. The estimated concentration of test item required to
produce a S.I. of 3 is referred to as the EC3 value.
EC3: 25.1 %
A dose-response relationship was observed. A calculation of the EC3
value was performed using the indicated concentrations (*).
In conclusion, the test item was found to be a skin sensitizer and an
EC3 value of 25.1% (w/v) was derived.
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