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EC number: 500-464-9 | CAS number: 160901-27-9 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP guideline study. According to the ECHA guidance document “Practical guide 6: How to report read-across and categories (March 2010)”, the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- (1997)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Fatty alcohol ether sulfate, sodium salt, C8-10 2EO
- IUPAC Name:
- Fatty alcohol ether sulfate, sodium salt, C8-10 2EO
- Test material form:
- semi-solid (amorphous): gel
- Remarks:
- migrated information: paste
Constituent 1
Method
- Target gene:
- his-operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Additional strain / cell type characteristics:
- other: All strains are deep rough and have a reduced capability to repair DNA-damage (except for TA 102). Strains TA 98, TA 100 and TA 102 contain the R-factor plasmid pkM101 enhancing an error prone DNA repair system.
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix prepared from the livers of rats treated with phenobarbital/beta-naphthoflavone was achieved from Trinova Biochem GmbH and was cofactor supplemented.
- Test concentrations with justification for top dose:
- plate incorporation and preincubation test: 3.16, 10.0, 31.6, 100, 316, 1000, 2500, 5000 µg/plate
- Vehicle / solvent:
- - Vehicle used: distilled water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: -S9 mix: Sodium azide: 10 µg/plate (TA 100, TA 1535), 4-NOPD: 10 µg/plate (TA 98)/40 µg/plate (TA1537), MMS: 1 µL/plate (TA 102); +S9 mix: 2-AA: 2.5 µg/plate (TA 98, TA 100, TA 1535, TA 1537) / 10 µg/plate (TA 102)
- Details on test system and experimental conditions:
- A preliminary plate incorporation experiment with the tester strains TA 98 and TA 100 was performed. The results were were in accordance with the acceptance criteria and are therfore reported as part of Experiment I.
METHOD OF APPLICATION:
Experiment I: in agar (plate incorporation);
Experiment II: preincubation
The initial plate incorporation test was conducted as follows:
Briefly, 0.1 mL test substance, 0.1 mL bacteria culture, 0.5 mL S9 mix (or buffer) and 2.0 mL soft agar were mixed and plated onto petri dishes with solid agar. After incubation at 37 °C for 48 h colonies were counted using an automatic counter. Each experiment was performed in triplicate.
The independent repeat was performed as preincubation test. Briefly, 0.1 mL test substance, 0.1 mL bacteria culture and 0.5 mL S9 mix (or buffer) were preincubated at 37 °C for 60 min. At the end of the preincubation period 2 mL of molten soft agar was added. After mixing, the suspension was plated, incubated for 48 h at 37 °C and colonies were counted using an automatic counter. Each experiment was performed in triplicate.
DETERMINATION OF CYTOTOXICITY
The reduction of background growth of bacteria on the plates as well as a reduction in the mutant count per plate to approximately 0.5 in comparison to control were used as marker for cytotoxicity. - Evaluation criteria:
- The following criteria determined the acceptance of an assay:
1. The negative controls had to be within the expected range, as defined by the laboratories’ own historical data.
2. The positive controls had to show sufficient effects, as defined by the laboratories' experience
3. The bacteria strains TA 98, TA 100 and TA 102 demonstrate their typical response to ampicillin.
4. Corresponding background growth on negative control, solvent control and test plates is oberved
A clear and dose-related increase in mutant counts of at least one strain is considered to be a positive result. For TA 98, TA 100, and TA 102 this increase should be about twice that of negative controls. For TA 1535 and TA 1537 the increase should be about three times than that of solvent controls. Otherwise, the result is evaluated as negative. However, these criteria may be overruled by good scientific judgment. - Statistics:
- Mean values and standard deviation were calculated.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- beginning at 1000 µg/plate in the plate incorporation experiment and 316 µg/plate in the preincubation experiment.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- No precipitation was observed in any tester strain at any concentration.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Test results of experiment 1 (plate incorporation).
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
||||
TA 100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|
without S9-Mix |
|||||
0 |
107 ± 7.5 |
6 ± 3.1 |
175 ± 10.6 |
25 ± 10.4 |
9 ± 2.5 |
3.16 |
128 ± 14.6 |
10 ± 6.7 |
196 ± 13.1 |
27 ± 6.4 |
14 ± 4.5 |
10.0 |
134 ± 4.2 |
7 ± 0.6 |
174 ± 13.4 |
21 ± 3.1 |
12 ± 3.1 |
31.6 |
134 ± 17.3 |
8 ± 1.7 |
185 ± 25.1 |
25 ± 2.5 |
9 ± 0.6 |
100 |
120 ± 15.0 |
8 ± 0.0 |
164 ± 15.1 |
28 ± 7.5 |
12 ± 2.5 |
316 |
95 ± 8.5 |
10 ± 3.2 |
182 ± 15.1 |
27 ± 9.7 |
8 ± 2.5 |
1000 |
74 ± 13.2 B |
10 ± 4.2 |
215 ± 10.8 |
21± 3.1 |
9± 4.2 |
2500 |
38 ± 3.0 B |
0± 0.0 B |
179± 10.8 |
19± 6.5 B |
6± 4.6 B |
5000 |
0± 0.6 B |
0± 0.0 B |
148± 11.0 |
14± 2.5 B |
0± 0.0 B |
Positive controls: |
Na-azide |
MMS |
4-NOPD |
||
Concentration (μg/plate) |
10 |
1 µL/plate |
10 |
40 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
934 ± 22.0 |
758 ± 220.3 |
1782 ± 345.7 |
461 ± 23.8 |
119 ± 7.8 |
With S9-Mix |
|||||
0 |
124 ± 13.8 |
7 ± 4.0 |
253 ± 5.3 |
26 ± 1.0 |
7 ± 3.5 |
3.16 |
120 ± 20.6 |
7 ± 7.1 |
272 ± 21.0 |
35 ± 5.2 |
11 ± 2.5 |
10.0 |
120 ± 8.1 |
10 ± 3.6 |
262 ± 2.5 |
35 ± 2.9 |
13 ± 4.5 |
31.6 |
113 ± 12.5 |
10 ± 3.2 |
262 ± 7.2 |
40 ± 7.2 |
10 ± 3.5 |
100 |
133 ± 16.7 |
7 ± 1.7 |
248 ± 19.7 |
35 ± 7.6 |
9 ± 1.0 |
316 |
121 ± 4.6 |
9 ± 1.0 |
277 ± 7.8 |
32 ± 3.5 |
12 ± 1.2 |
1000 |
117 ± 29.5 |
11 ± 1.7 |
274 ± 7.0 |
36 ± 5.3 |
13 ± 3.8 |
2500 |
100 ± 9.9 |
7 ± 1.5 |
233 ± 8.4 |
31 ± 3.1 |
6 ± 2.5 |
5000 |
61 ± 4.4 B |
4 ± 1.0 B |
238 ± 28.6 |
20 ± 5.5 |
9 ± 1.5 |
Positive controls: |
2-AA |
||||
Concentrations (μg/plate) |
2.5 |
10 |
2.5 |
||
Mean No. of colonies/plate (average of 3 ± SD) |
1581 ± 229.7 |
102 ± |
516 ± |
1959 ± 504.5 |
261 ± |
MMS = Methylmethanesulfonate
4-NOPD = 4-Nitro-o-phenylene diamine
2-AA = 2-Aminoanthracene
B = Reduction of background lawn
Table 2: Test results of experiment 2 (pre-incubationtion).
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
||||
TA 100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|
without S9-Mix |
|||||
0 |
115± 23.1 |
10± 4.0 |
270± 12.2 |
22± 3.2 |
7± 1.5 |
3.16 |
129± 14.4 |
4± 2.1 |
241± 29.2 |
24± 1.2 |
10± 2.0 |
10.0 |
133± 9.0 |
9± 2.3 |
264± 29.4 |
31± 2.5 |
10± 6.0 |
31.6 |
119± 4.4 |
5± 1.0 |
278± 29.7 |
23± 2.6 |
12± 6.4 |
100 |
114± 15.9 |
8± 2.0 |
283± 23.1 |
26± 1.0 |
9± 3.2 |
316 |
100± 6.1 |
7± 1.2 |
262± 9.8 |
25± 2.1 |
8± 1.2 B |
1000 |
58± 9.1 B |
0± 0.0 B |
280± 22.8 |
25 ± 0.6 B |
0 ± 0.0 B |
2500 |
0 ± 0.0 B |
0 ± 0.0 B/N |
155 ± 37.0 |
22 ± 6.1 B |
0 ± 0.0 N |
5000 |
0 ± 0.0 N |
0 ± 0.0 N |
125 ± 45.0 B |
0 ± 0.0 B |
0 ± 0.0 N |
Positive controls: |
Na-azide |
MMS |
4-NOPD |
||
Concentration (μg/plate) |
10 |
1 µL/plate |
10 |
40 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
965± 120.2 |
1038± 227.1 |
2158± 67.5 |
389± 30.1 |
107± 7.0 |
With S9-Mix |
|||||
0 |
108± 12.7 |
10± 2.5 |
292± 7.8 |
38± 4.5 |
9± 3.2 |
3.16 |
137± 30.7 |
5± 2.1 |
289± 15.9 |
39± 5.6 |
7± 2.1 |
10.0 |
139± 4.2 |
5± 2.5 |
323± 36.5 |
37± 2.0 |
4± 2.9 |
31.6 |
121± 14.0 |
7± 3.1 |
310± 21.2 |
32± 4.5 |
5± 4.9 |
100 |
134± 14.7 |
7± 1.5 |
297± 6.1 |
31± 6.0 |
7± 2.6 |
316 |
116± 7.6 |
8± 1.5 |
286± 24.0 |
31± 6.7 |
4 ± 1.2 |
1000 |
101± 15.5 |
7± 0.6 |
310± 7.4 |
30± 12.7 |
12± 3.5 |
2500 |
85± 11.8 B |
4± 2.5 B |
284± 15.0 |
22± 4.2 B |
7± 3.5 B |
5000 |
70± 4.6 B |
0± 0.0 B |
273± 10.7 |
21± 10.4 B |
6± 3.6 B |
Positive controls: |
2-AA |
||||
Concentrations (μg/plate) |
2.5 |
10 |
2.5 |
||
Mean No. of colonies/plate (average of 3 ± SD) |
789± |
60 ± |
628 ± |
2943 ± |
128 ± |
MMS = Methylmethanesulfonate
4-NOPD = 4-Nitro-o-phenylene diamine
2-AA = 2-Aminoanthracene
B = Reduction of background lawn
N = No background lawn
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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