Reaction mass of Methylium, tris[4-(diethylamino)phenyl]- & acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :1. and 2. Short term toxicity of test material to aquatic invertebrates was performed according to the OECD 202 guideline in a static system.

GLP compliance:

not specified

Specific details on test material used for the study:

IUPAC name: Reaction mass of Methylium, tris[4-(diethylamino)phenyl]- & acetateMolecular weight: 515.7375Molecular formula: C33H45N3O2Smiles: CCN(CC)c1ccc(C{+}(c2ccc(N(CC)CC)cc2)(c2ccc(N(CC)CC)cc2).O{-}C(C)=O)cc1Inchl: 1S/C31H42N3.C2H4O2/c1-7-32(8-2)28-19-13-25(14-20-28)31(26-15-21-29(22-16-26)33(9-3)10-4)27-17-23-30(24-18-27)34(11-5)12-6;1-2(3)4/h13-24H,7-12H2,1-6H3;1H3,(H,3,4)/q+1;/p-1Physical state: Solid and liquidForm: Organic

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

1.The stock solution 20 mg/L was prepared by dissolving dark violet iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.2.The stock solution (10 mg/L) was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: Water flea- Strain: Straus- Source: Own breeding at University of Chemistry and Technology, Prague- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny- Feeding during test: No feeding

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

20±1°C

pH:

1. and 2.) Test at 0.100 mg/l: 8.1 (changed to 7.9 during test)Control: 7.7 (Changed to 7.9 during test)

Nominal and measured concentrations:

1) 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l2) 0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L

Details on test conditions:

1 and 2) TEST SYSTEM- Test vessel: 50 ml glass vessel- fill volume: 25 ml- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.As an example, the preparation of dilution water meeting the requirements is described below.Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.Prepare the solutions specified below:- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).MixingMix 2.5 ml of each of the four solutions and make up to 1 l with water.The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.- Hydrochloric acid, e.g. [HCl] : 1 mol/l.Reference substance: Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).OTHER TEST CONDITIONS- Adjustment of pH: no adjustment done- Photoperiod: No - Darkness- Light intensity:CALCULATION:EC50 was calculated using non linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.037 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.025-0.054 mg/l for 1st study

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.116 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.098 - 0.138 mg/L for 2nd study

Results with reference substance (positive control):

- Results with reference substance valid- EC50: 0.76 mg/L (24 hours)

Reported statistics and error estimates:

EC50 was calculated using non linear regression by the software Prism 4.0

Validity criteria fulfilled:

not specified

Conclusions:

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L on the basis of mobiity inhibition effects in a 48 hour study. 2. The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be 0.116 mg/L on the basis of mobiity inhibition effects in a 48 hour study. Thus, based on the above summarised studies, Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate it can be concluded that effect concetration value is in the range of 0.03 - 0.116 mg/ L. Thus, comparing this value with the criteria of CLP regulation Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be classified for as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Executive summary:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate. The studies are as mentioned below:

1.Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The stock solution 20 mg/L was prepared by dissolving dark violet iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 as per the CLP criteria.

2.Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The stock solution (10 mg/L) was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.016 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as Aquatic acute category 1 as per the CLP criteria.

Thus, based on the above summarised studies, Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate it can be concluded that effect concetration value is in the range of 0.03 - 0.116 mg/ L. Thus, comparing this value with the criteria of CLP regulation Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be consider as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Description of key information

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

2. The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be 0.116 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

Thus, based on the above summarised studies, Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate it can be concluded that effect concetration value is in the range of 0.03 - 0.116 mg/ L. Thus, comparing this value with the criteria of CLP regulation Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be classified for as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.037 mg/L

Additional information

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate. The studies are as mentioned below:

In the key study from the experimental report 2016 toxicity was determine. Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The stock solution 20 mg/L was prepared by dissolving dark violet iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 as per the CLP criteria.

Similarly the first study was supported by the second. Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The stock solution (10 mg/L) was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.016 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as Aquatic acute category 1 as per the CLP criteria.

Thus, based on the above summarised studies, Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate it can be concluded that effect concetration value is in the range of 0.03 - 0.116 mg/ L. Thus, comparing this value with the criteria of CLP regulation Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be consider as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.