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Biodegradation in water

Estimation Programs Interface Suite was run to predict the biodegradation potential of the test compound  Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate is expected to be not readily biodegradable.

Additional information

Biodegradation in water

Predicted data for the target compound Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate and various supporting weight of evidence studies for its structurally and functionally similar read across substance were reviewed for the biodegradation end point which are summarized as below:

 

In a prediction done using the Estimation Programs Interface Suite was run to predict the biodegradation potential of the test compound Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate is expected to be not readily biodegradable.

 

In a supporting weight of evidence study from study report (2018),28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 107 to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 73.49%. Degradation of Sodium Benzoate exceeds 31.32% on 7 days & 49.39% on 14th day. The activity of the inoculum is thus verified and the test can be considered as valid. The BOD35 value of test chemical was observed to be 0.52 mgO2/mg. ThOD was calculated as 2.31 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 22.51%. Based on the results, the test item, under the test conditions, was not readily biodegradable in nature.

 

For the test chemical,Biodegradation study was conducted for evaluating the percentage biodegradability of test substance(Toshihide Saito et. al., 1984). Activated sludge was used as a test inoculum obtained from municipal sewage. Test substance of conc. 500 mg/l was prepared and diluted as needed. The COD measurement was performed by the potassium dichromate reflux method based on Japanese Industrial Standards. In the BOD measurement, a sample solution was taken into a container. Then JIS-BOD testing solutions, i.e. 3 ml of A solution and 1 ml of B, C, D solutions were added to the sample solution, respectively. Then 5 ml of the supernatant of the municipal sewage activated sludge was added to the sample solution and diluted to 300 ml with water. Immediately, the BOD-time curve was recorded at 20~ using an Ohkura OM-200I type coulometer. The TOC measurement was carried out by a Yanagimoto TOC-ILW.BOD5, COD, ThOD and TOC value of the test chemical was determined to be 0.038, 1.73, 2.88 and 0.50 g/g, respectively. The BOD5/TOC ratio of test chemical was determined to be 0.08 (i.e. ranges in between 0.08-0.89), indicating that the test chemical is highly resistant to aerobic biodegradation. Thus, based on this value BOD5/TOC value, it can be concluded that test chemical is not readily biodegradable in nature.

 

In a supporting weight of evidence study from peer reviewed journal (H. Heukelekian et. al., 1955),Biodegradation experiment was conducted for 5 days for evaluating the percentage biodegradability of test substance using standard dilution method under aerobic conditions at a temperature of 20°C. Sewage was used as a test inoculum. The 5 day BOD value of test chemical was determined to be 0 g/g. Thus, based on this value, test chemical is considered to be not readily biodegradable in nature.

 

On the basis of above results for target chemical Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate (from modelling database, 2018), it can be concluded that the test substance Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be expected to be not readily biodegradable in nature.