Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the OECD guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203.
GLP compliance:
not specified
Specific details on test material used for the study:
IUPAC name: Reaction mass of Methylium, tris[4-(diethylamino)phenyl]- & acetate Molecular weight: 515.7375Molecular formula: C33H45N3O2Smiles: CCN(CC)c1ccc(C{+}(c2ccc(N(CC)CC)cc2)(c2ccc(N(CC)CC)cc2).O{-}C(C)=O)cc1Inchl: 1S/C31H42N3.C2H4O2/c1-7-32(8-2)28-19-13-25(14-20-28)31(26-15-21-29(22-16-26)33(9-3)10-4)27-17-23-30(24-18-27)34(11-5)12-6;1-2(3)4/h13-24H,7-12H2,1-6H3;1H3,(H,3,4)/q+1;/p-1Physical state: Solid and liquidForm: Organic
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of potable water (passed through reverse osmosis system) with continuous 1 hr stirring. After stirring stock was analytically detected and the solubility was found 672.34 mg/L, on the basis of this value following test concentration was prepared 0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name: Zebra fish(Danio rerio)- Source: Anytime Pet, Jariphataka, Nagpur- Length at study initiation (length definition, mean, range and SD): average 1.29 cm- Weight at study initiation (mean and range, SD): 0.049g (average)ACCLIMATION- Acclimation period: 7 days- Acclimation conditions (same as test or not): Same as test- Feeding frequency during acclimation: Daily once- Health during acclimation (any mortality observed): No mortality were observedOther conditions during acclimation: - Photoperiod 16 hours light and 8 hours dark- Temperature range: 24.1 °C- pH range 7.1- DO range 7.0 mg/l- Aeration Continous
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Post exposure observation period:
24, 48, 72, 96 hrs
Hardness:
155.5 (mg of CaCO3)
Test temperature:
23 °C
pH:
7.2
Dissolved oxygen:
7 mg/l
Nominal and measured concentrations:
0.1562mg/L, 0.3125mg/L, 0.625mg/L, 1.25mg/L and 2.5mg/L
Details on test conditions:
TEST SYSTEM - Test vessel: Plastic aquaria - Material, size, headspace, fill volume: Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water with 3 liter of headspace. - Aeration: Aeration in test vessels was provided 1 day before the start of experiment - No. of organisms per vessel: 8 - No. of vessels per concentration (replicates): 2 OTHER TEST CONDITIONS - Adjustment of pH: No - Photoperiod: 16 hours light and 8 hours dark EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations of (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
< 0.156 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Toxic to fish
Key result
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
0.156 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Other details not known

Fig:Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.1562

4

4

-

-

-

-

4

4

4

4

4

4

4

4

0.3125

4

4

-

-

-

-

4

4

4

4

4

4

4

4

0.625

4

4

-

-

4

4

4

4

4

4

4

4

4

4

1.25

4

4

-

-

4

4

4

4

4

4

4

4

4

4

2.5

4

4

4

4

4

4

4

4

4

4

4

4

4

4

Fig: Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

4

4

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

-

-

-

-

-

-

0.1562

4

4

M4

M4

M4

M4

-

-

-

-

-

-

-

-

0.3125

4

4

M4

M4

M4

M4

-

-

-

-

-

-

-

-

0.625

4

4

M4

M4

-

-

-

-

-

-

-

-

-

-

1.25

4

4

M4

M4

-

-

-

-

-

-

-

-

-

-

2.5

4

4

IS4

IS4

-

-

-

-

-

-

-

-

-

-

          

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

 

 

Fig: pH and oxygen content:

 

 

Number of

pH readings after

 

 

 

 

Nominal

fish

24h

 

48h

 

72h

 

96h

 

Concentration

 

 

V1

V2

V1

V2

V1

V2

V1

V2

(mg/l)

 

 

 

 

 

 

 

 

 

 

Control

4

4

-

-

-

-

-

-

-

-

0.1562

4

4

-

-

-

-

-

-

-

-

0.3125

4

4

-

-

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

1.25

4

4

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

0.1562

4

4

-

-

-

-

-

-

-

-

0.3125

4

4

-

-

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

1.25

4

4

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

8.3. Test temperature

 

 

 

 

 

 

 

 

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

0.1562

4

4

-

-

-

-

-

-

-

-

0.3125

4

4

-

-

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

1.25

4

4

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetat on Zebra fish (Danio rerio) was determined to be < 0.1562 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic.
Executive summary:

This study was designed to assess the toxic effects of the test Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate on the Zebra fish (Danio rerio). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of potable water (passed through reverse osmosis system) with continuous 1 hr stirring. After stirring stock was analytically detected and the solubility was found 672.34 mg/L, on the basis of this value following test concentration was prepared 0.1562mg/L, 0.3125mg/L, 0.625mg/L, 1.25mg/L,2.5mg/L ,respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate on Zebra fish (Danio rerio) was determined to be < 0.1562 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic acute 1 chronic 1 as per the CLP criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetat on Zebra fish (Danio rerio) was determined to be < 0.1562 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic.

Key value for chemical safety assessment

LC50 for freshwater fish:
1 562 mg/L

Additional information

This study was designed to assess the toxic effects of the test compound Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate on the Zebra fish (Danio rerio). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of potable water (passed through reverse osmosis system) with continuous 1 hr stirring. After stirring stock was analytically detected and the solubility was found 672.34 mg/L, on the basis of this value following test concentration was prepared 0.1562mg/L, 0.3125mg/L, 0.625mg/L, 1.25mg/L,2.5mg/L ,respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate on Zebra fish (Danio rerio) was determined to be < 0.1562 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic acute 1 chronic 1 as per the CLP criteria.