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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
None
GLP compliance:
yes
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
All test medium samples for the analytical determinations were taken after mixing of the test solutions.
Vehicle:
yes
Remarks:
Bidistilled water
Details on test solutions:
Test concentrations
Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/l nominal.
Test organisms (species):
Daphnia magna
Details on test organisms:
The test organism was the species Daphnia magna. The daphnids were bred in the laboratories of the Textile Dyes Division, Product Ecology TF under standardized conditions. For the test, animals with age of less than 24 hours were used. The quality of the daphnids will be checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate (range:0.8 -1.5 mg/l).
For each concentration 20 Daphnia magna were used.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
136mg/l CaC03
Test temperature:
21 ± 1 °C
pH:
7.8 - 8.2.
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/l nominal.
Details on test conditions:
48 hours at 21 ± 1 °C, 16 h light/ 8 hours dark The daphnids were exposed to the test article in 250 ml beakers containing 100 ml test medium with 10 animals per beaker. All tests are running in duplicate. During exposure, the animals were kept at 21 ± 1 °C with 16 hours illumination.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 179.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 179.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
>= 179.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
None
Results with reference substance (positive control):
The EC50 value for the reference substance used was determined as 0.93 mg/l.
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
yes
Conclusions:
The 48 hour EC50 of FAT 40000 was determined to be >179.1 mg/L.
Executive summary:

The acute toxicity of FAT 40000/G to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemical, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested were 58, 100 and 180 mg test article/I, and a control without any additions. No immobility was observed during test duration in the control. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 99.1 - 104.7 % of the nominal values. Therefore, under the test conditions the test article was sufficiently stable.

In the control no immobilization of the daphnids was observed after 48 hours. In the lowest and in the highest test concentration of 60.6 mg/l and 179.1 mg/l, respectively, no toxic effect on the daphnids was found. Only in the test concentration of 102.0 mg/l one daphnia was immobilized. But this effect is probably not caused by the test substance. Therefore, the EC50 values could not be calculated. The EC0 value is at least 179.1 mg/l, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 179.1 mg/l.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that the source and the target substance have very similar physicochemical and (eco)toxicological properties because their chemical structures are nearly identical. An analogue approach has thus been employed. The target substance is the meta-isomer of the dye Reactive Yellow 095, where the sulphonate group is bound at the meta-position of the amino benzene moiety. The source chemical is the reaction mass of both the meta-isomer and the para-isomer of Reactive Yellow 095.
The presence of sulphonate groups make both dyes highly water soluble and therefore less critical for human health and environmental issues. Based on their chemical similarity, similar properties are expected in both humans and the environment.
2. SOURCE AND TARGET CHEMICAL(S)
Source: Reactive Yellow 095 meta/para (CAS# --- / EC# 944-218-2)
Target: Reactive Yellow 095 meta (CAS# 84045-63-6 / EC# 281-865-3)
3. ANALOGUE APPROACH JUSTIFICATION
see attachment under 4.12 Auto flammability
Reason / purpose:
read-across source
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 179.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 179.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 179.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Conclusions:
The 48h EC50 in Daphnia magna was determined to be >179.1 mg/L
Executive summary:

The acute toxicity of the source substance to Daphnia magna was determined in a 48-hour static test according to the OECD TG 202. The nominal concentrations tested were 58, 100 and 180 mg/L, and a control without any additions. No immobility was observed during test duration in the control. The reported results are related to the analytical concentrations of the test article.

In the control, no immobilization of the daphnids was observed after 48 hours. In the lowest and in the highest test concentration of 60.6 mg/l and 179.1 mg/l, respectively, no toxic effect on the daphnids was found. Only in the test concentration of 102.0 mg/l one daphnia was immobilized. But this effect is probably not caused by the test substance.

The structurally related target substance will show the same behaviour and therefore it can be anticipated that the 48h-EC50 will be at least >100 mg/L as well.

Description of key information

The 48-hour EC50 in Daphnia magna was determined to be >179 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
179 mg/L

Additional information

The acute toxicity of the source substance to Daphnia magna was determined in a 48-hour static test according to the OECD TG 202. The nominal concentrations tested were 58, 100 and 180 mg/L, and a control without any additions. No immobility was observed during test duration in the control. The reported results are related to the analytical concentrations of the test article.

In the control, no immobilization of the daphnids was observed after 48 hours. In the lowest and in the highest test concentration of 60.6 mg/l and 179.1 mg/l, respectively, no toxic effect on the daphnids was found. Only in the test concentration of 102.0 mg/l one daphnia was immobilized. But this effect is probably not caused by the test substance.

The structurally related target substance will show the same behaviour and therefore it can be anticipated that the 48h-EC50 in Daphnids will be >179 mg/L as well.