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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1973
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
None
Qualifier:
according to
Guideline:
other: CIBA Method A-11
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
yes
Details on test solutions:
Water
Test organisms (species):
other: trout and goldorfe
Details on test organisms:
None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
None
pH:
None
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
None
Details on test conditions:
None
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Remarks:
TL0
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
trout
Duration:
96 h
Dose descriptor:
LC0
Remarks:
TL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
goldorfe
Key result
Duration:
96 h
Dose descriptor:
LC50
Remarks:
TL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Remarks:
TL100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
None
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 (TL) of FAT 40000 in this fish acute toxicity test was determined to be greater than 1000 mg/L.
Executive summary:

An acute fish toxicity test was performed with trout and goldorfe. Groups of fish were exposed at the test concentrationsof 100, 200 and 1000 mg/L. Based on the findings, the LC0 value was 200 mg/l (trout) or 1000 mg/l (goldorfe), whereas LC50 and LC100 value was determined to be greater than 1000 mg/l in both species.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
None
Qualifier:
according to
Guideline:
other: CIBA Method A-11
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
yes
Details on test solutions:
Water
Test organisms (species):
Salmo sp.
Details on test organisms:
None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
12 °C
pH:
8
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
100, 200 and 1000 mg/l
Details on test conditions:
None
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Remarks:
TL0
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Remarks:
TL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Remarks:
TL100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
None
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 (TL) of FAT 40000/A in this fish acute toxicity test was determined to be greater than 1000 mg/L.
Executive summary:

An acute fish toxicity test was performed with trout. Groups of fish were exposed at the test concentrationsof 100, 200 and 1000 mg/L. Based on the findings, the LC0, LC50 and LC100 (TL) value was determined to be greater than 1000 mg/l.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
None
Qualifier:
according to
Guideline:
other: CIBA Method A-11
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
yes
Details on test solutions:
Water
Test organisms (species):
other: Red killi and trout
Details on test organisms:
None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
None
pH:
None
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
None
Details on test conditions:
None
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Remarks:
TL0
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Remarks:
TL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Remarks:
TL100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
None
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 (TL) of FAT 40000/C in this fish acute toxicity test was determined to be greater than 1000 mg/L.
Executive summary:

An acute fish toxicity test was performed with Red killi fish and trout. Based on the findings, the LC0, LC50 and LC100 (TL) value was determined to be greater than 1000 mg/l for both fish species.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
None
Qualifier:
according to
Guideline:
other: CIBA Method A-11
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
yes
Details on test solutions:
Water
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
25 °C
pH:
10
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
300 and 1000 mg/l
Details on test conditions:
None
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
None
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of FAT 40000/D in this fish acute toxicity test was determined to be greater than 1000 mg/L.
Executive summary:

An acute fish toxicity test was performed with Zebra-fish ( Brachydanio rerio ). Groups of fish were exposed at the test concentrationsof 300 and 1000 mg/L. Based on the findings, the LC0, LC50 and LC100 value was determined to be greater than 1000 mg/l.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
None
Qualifier:
according to
Guideline:
other: CIBA Method A-11
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
yes
Details on test solutions:
Water
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
23 °C
pH:
10
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
300 and 1000 mg/l
Details on test conditions:
None
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
None
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of FAT 40000/E in this fish acute toxicity test was determined to be greater than 1000 mg/L.
Executive summary:

An acute fish toxicity test was performed with Zebra-fish (Brachydanio rerio). Groups of fish were exposed at the test concentrationsof 300 and 1000 mg/L. Based on the findings, the LC0 value was determined to be 300 mg/l. The LC50 and LC100 value was determined to be greater than 1000 mg/l.

Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that the source and the target substance have very similar physicochemical and (eco)toxicological properties because their chemical structures are nearly identical. An analogue approach has thus been employed. The target substance is the meta-isomer of the dye Reactive Yellow 095, where the sulphonate group is bound at the meta-position of the amino benzene moiety. The source chemical is the reaction mass of both the meta-isomer and the para-isomer of Reactive Yellow 095.
The presence of sulphonate groups make both dyes highly water soluble and therefore less critical for human health and environmental issues. Based on their chemical similarity, similar properties are expected in both humans and the environment.
2. SOURCE AND TARGET CHEMICAL(S)
Source: Reactive Yellow 095 meta/para (CAS# --- / EC# 944-218-2)
Target: Reactive Yellow 095 meta (CAS# 84045-63-6 / EC# 281-865-3)
3. ANALOGUE APPROACH JUSTIFICATION
see attachment under 4.12 Auto flammability
Reason / purpose:
read-across source
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not specified
Conclusions:
The 96h-LC50 in zebrafish was >1000 mg/L.
Executive summary:

Based on one key study and four supporting studies for the source substance, it was clear that the 96h-LC50 in fish was >1000 mg/L. The structurally related target substance will show the same behaviour, and the 96h-LC50 will be as well >1000 mg/L.

Description of key information

The LC50 in fish was determined to be greater than 1000 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
1 000 mg/L

Additional information

A key study to determine acute toxicity to fish was performed with Zebra-fish (Brachydanio rerio). Groups of fish were exposed at the test concentrations of 300 and 1000 mg/L. Based on the findings, the LC0 values was 300 mg/L, whereas LC50 and LC100 value was determined to be greater than 1000 mg/L. Several supporting studies conducted on different batches of this test substance and involving different fish species confirmed this test result indicating that the effects are not dependent on fish species or production batch.

The structurally related target substance will show the same behaviour and therefore it can be anticipated that the 96h-LC50 in fish will be >1000 mg/L as well.