Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-369-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-01-29 to 2020-03-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 10, 31.6, 100, 316, 1000 mg active acid/kg soil dry weight
- Sampling method: solid phase extraction, then photometric determination
- Sample storage conditions before analysis: Not reported - Vehicle:
- no
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: A field fresh, silty sand soil (LUFA standard soil 2.3) was used in the study. Nominal soil parameters were:
Sand content 50 - 75 %
pH value 5.5 - 7.5
Organic carbon content 0.5 - 1.5 %
Microbial biomass (expressed as carbon content) at least 1 % of the total soil organic carbon.No crop protection products were used for a minimum of one year before sampling. No organic fertiliser was applied for at least six months prior use.
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: The test item was weighed out and diluted in demineralized water for each test concentration separately and applied to the surface of the soil. The soil was mixed thoroughly with a mixer (approximately 3 minutes) to ensure a homogeneous distribution of the test item in the soil. Afterwards, the soil was distributed to the replicates.
VEHICLE: N/a - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 ± 2 °C
- Moisture:
- At experimental starting the pre-moistened soil was adjusted to approximately 45 % of its maximum water holding capacity. All replicates were weighed once per week to check for water losses by evaporation (recommended maximum 5 %, actual 0.2 - 1.5 %). Compensation with demineralized water was done. Replicates were weighed for this procedure.
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Noack Laboratorien GmbH, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
- Test container (type, material, size): Plastic boxes (volume 1.0 L, food grade) with perforated tops to enable gas exchange. Incubation was performed in bulk and sub-samples were taken.
- Amount of soil: 450 g soil dry weight
- No. of replicates per concentration: 3
- No. of replicates per control: 3
- No. of replicates per vehicle control: n/a
SOIL INCUBATION
- Method: bulk / series of individual subsamples
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude):
- History of site:
- Vegetation cover:
- Treatments with pesticides or fertilizers:
- Accidental contamination:
- Other:
- Depth of sampling: 0-20 cm
- Soil texture: silty sand (uS)
- % sand: approx. 58%
- % silt: approx. 35%
- % clay: approx. 7.3%
- Soil taxonomic classification: LUFA soil
- Soil classification system: LUFA soil 2.3, Batch number F2.31820
- pH (in water): 6.1 +/- 0.4
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight):
- Maximum water holding capacity (in % dry weight):35.2 ± 1.8g/100g DW
- Cation exchange capacity (mmol/kg): 6.8 ± 1.4 meq / 100 g
- Pretreatment of soil: The soil amounts were amended with powdered lucerne-green-grass-meal (0.5 % of soil dry weight).
Total nitrogen content: 24.6 g/kg
Total carbon content: 43.9 %
C/N ratio: 17.8
- Storage (condition, duration): The soil was stored for 2 days (2020-05-05 to 2020-05-07) in the dark at 4 - 6 °C in a climatic room. Subsequently, the soil was pre-incubated at room temperature (ca. 20 °C) for 21 days
(2020-05-07 to 2020-05-28) to adapt the micro-organisms to test conditions before the start of the study.
- Initial microbial biomass as % of total organic C: 2.26%
DETAILS OF PREINCUBATION OF SOIL (if any):N/a
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Nitrate-N content increase compared toc ontrol: every 7 days. Nitrate-N formation rate at 7d, 14d, and 28d
VEHICLE CONTROL PERFORMED: n/a
RANGE-FINDING STUDY
- Test concentrations: 0, 100, 1000 mg active acid/kg dry weight
- Results used to determine the conditions for the definitive study: yes - Nominal and measured concentrations:
- Nominal: control, 10, 31.6, 100, 316, 1000 mg active acid/kg soil dry weight
- Reference substance (positive control):
- yes
- Remarks:
- Cyanoguanidine
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 54.9 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: 95% C.I. <10 - 128
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 802 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: 95% C.I. 558 - >1000
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: No
- Other: N/a - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: at 25 mg/kg soil dry weight, 28d of 32% difference to control in Nitrate-N, 39% in nitrate-n formation rate
- Other: - Reported statistics and error estimates:
- ECx values were calculated by sigmoidal dose-response regression using GraphPadPrism. Four parameter were used for dose-response fitting. Calculation of the confidence intervals for ED-values was carried out using standard procedures provided by GraphPadPrism.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 28d EC10 value for the effects of HMDTMP(4-7K) on the nitrogen formation rate of soil microorganisms is 54.9 mg active acid/kg dry weight (95% C.I of <10 - 128)
Reference
Increase of Nitrate-N formation rate in soil containing HMDTMP(4 -7K) aqueous solution, relative to the control.
Nitrate-N Formation Rate | |||
Test item concentration | Increase [%] compared to control | ||
[mg a.i./kg DW] | 7 d | 14 d | 28 d |
10.0 | 33 | 13 | 4 |
31.6 | 71 | 31 | 14 |
100 | 61 | 31 | 7 |
316 | 65 | 30 | 36 |
1000 | 76 | 78 | 52 |
Description of key information
28d EC10 54.9 mg active acid/kg dry weight, reliability 1, soil microorganisms (read-across from HMDTMP(4-7K), Noack, 2020).
This study is currently in draft format at the moment, however the values are not expected to change in the final study report.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 54.9 mg/kg soil dw
Additional information
A 28-d EC10 value of 54.9 mg active acid/kg soil dry weight has been determined for the effects of HMDTMP-(4-7K) on the nitrogen formation rate of soil microorganisms, based on nominal concentrations. An EC50 value of 802 mg active acid/kg soil dry weight based on nitrogen formation has also been reported in the same study (Noack, 2020).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Route: .live1