Slags, ferrochromium-manufg.

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented publication which meets basic scientific principles

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Glass dust: A study of its biologic effects

Author:

Gross P, M.L.Westrick

Year:

1960

Bibliographic source:

AMA Arch Ind Health 21:10-23

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Two experiments were performed, each using six eyes of albino rabbits. In the first, the lids were held open only long enough to dust the ocular conjunctiva thoroughly with the finely miIled glass flakes (the largest particles around 400 microm but the vast majority less than 50 microm). In the second experiment, the lids were held open about one minute after the dusting. The eyes were examined 24 hours later with the aid of fluorescein.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Glass

EC Number:

920-837-3

IUPAC Name:

Glass

Details on test material:

The glass dust used in this investigation prepared from flakes of glass (Fiberglas Flake) which were approximately 5 microm thick. The glass contained, in addition to SiO2, the oxides of Al, Ca, Mg, and B as major constitllents. AIso present, but in amounts less than 1'% and usually more than trace, were the followingelemellts: Na, K, Ti, Fe, and Zr.
A coarse dust was prepared by milling the flakes so that the resulting particles passed a 32S mesh sieve.
The largest particles were around 400 microm but the vast majority less than 50 microm

Test animals / tissue source

Species:

rabbit

Strain:

other: "albino"

Test system

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

Not specified. the lids were held open only long enough to dust the ocular conjunctiva thoroughly with the finely miIled glass ftakes (the largest
particles around 400microm but the vast majority less than 50microm)

Duration of treatment / exposure:

24h

Observation period (in vivo):

24h

Number of animals or in vitro replicates:

6

Details on study design:

Two experiments were performed, each using six eyes of albino rabbit!s. In the first, the lids were held open only long enough to dust the ocular conjunctiva thoroughly with the finely miIled glass flakes (the largest particles around 400 microm but the vast majority less than 50 microm). In the second experiment, the lids were held open about one minute after the dusting. The eyes were examined 24 hours later with the aid of fluorescein.

Results and discussion

In vivo

Results

Irritation parameter:

other: no corneal defects were observed

Basis:

animal #6

Time point:

other: 24 h

Score:

0

Irritant / corrosive response data:

No corneal defects were observed

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

The glass particles did not reveal any corneal effects.

Executive summary:

Glass particles (mostly less than 50 microm and upto 400 microm) did not show any corneal effetcts when applied to rabbit eyes (Gross et al. 1960). The particles were effectively cleared from conjunctiva by blinking.