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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from authorative database

Data source

Reference
Reference Type:
other: authorative database
Title:
Hazardous Substances Data Bank (HSDB) for 2-METHYL-1,4-BENZENEDIAMINE
Author:
U.S National Library of Medicine
Year:
2017
Bibliographic source:
Hazardous Substances Data Bank (HSDB) for 2-METHYL-1,4-BENZENEDIAMINE U.S National Library of Medicine 2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To evaluate eye irritation potential of 2,5-Di amino toluene on rabbit’s eye
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
-IUPAC name:2-methylbenzene-1,4-diamine
-Molecular formula :C7H10N2
-Molecular weight :122.17 g/mol
- Substance type:organic
- Physical state: solid
-InChI:1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
-Smiles:Cc1cc(ccc1N)N

Specific details on test material used for the study:
-IUPAC name:2-methylbenzene-1,4-diamine
-Molecular formula :C7H10N2
-Molecular weight :122.17 g/mol
- Substance type:organic
- Physical state: solid
-InChI:1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
-Smiles:Cc1cc(ccc1N)N

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
other: aqueous sodium sulphite (pH 7.0)
Amount / concentration applied:
0.1 mL of a 2.5% w/v
Duration of treatment / exposure:
10 seconds
Observation period (in vivo):
1 hr , Days 1, 2, 3, 4, and 7.
Number of animals or in vitro replicates:
Total=3
Details on study design:
Washing (if done): eye was rinsed with 50 mL of lukewarm water
SCORING SYSTEM: Draize scoring system

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
7 d
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Mild conjunctival irritation was observed in 2 animals on days 1 and 3 respectively

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
2,5-Diaminotoluene was installed into the eye of three rabbits for in the concentration 0.1 mL of a 2.5% w/v in aqueous sodium sulphite (pH 7.0) for 10 sec. Mild conjunctival irritation was observed in 2 animals on days 1 and 3 respectively. Hence, 2,5-Diaminotoluene was considered to be slightly irritating to rabbit eye.
Executive summary:

2,5-Diaminotoluene was used as a test material to evaluate ocular irritation potential on three rabbits. The test material was installed onto the eyes of rabbits in the concentration 0.1 mL of a 2.5% w/v in aqueous sodium sulphite (pH 7.0) for 10 sec. After 10 seconds the eye was rinsed with 50 mL of lukewarm water.eyes were evaluated and scored by Draize scoring system at 1 hr and then at Days 1, 2, 3, 4, and 7.

Mild conjuctival irritation was observed in 2 animals on day 1 and 3 respectively . No data regarding reversibility of the irritation was stated. Under the conditions of this test, toluene-2,5-diamine solution caused slight irritation to rabbit eyes.