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Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2). EC50  Intoxication value  was estimated to be 204.963 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2) was likely to be not toxic to aquatic invertebrate.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to algae was predicted 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2). EC50  Growth value value was estimated to be 109.835 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2) was likely to be not toxic to aquatic algae.

Additional information

Short-term toxicity to aquatic invertebrates

Following three studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 2-chlorobenzene-1,4-diammonium sulphate (CAS: 6219-71-2) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2). EC50  Intoxication value  was estimated to be 204.963 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2) was likely to be not toxic to aquatic invertebrate.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across 2-chlorobenzene-1,4-diammonium sulphate (CAS: 6219-71-2) from the publicationChemosphere2009, suggests that theShort term toxicity to aquatic invertebrates was performed in Daphnia magna (Water Flea) for 48 hrs with <24 hrs age and life stage Juvenile(s) with 2ND-5THBROOD

Test in fresh water media with static condition. During the experiment, the EC50 value for 4-amino-N-pyrimidin-2-ylbenzenesulfonamide (Cas no. 68-35-9) was determined to be 212 mg/l on the basis of mobility.

Based on the effect value test chemical N-pyrimidin-2-ylbenzenesulfonamide (Cas no. 68-35-9) was considered to be non toxic to aquatic invertebrates and thus considered to not classified as per the CLP classification criteria.

Another supporting experimental study for the structurally similar read across Sulfadimethoxine(CAS: 122 -11 -2) from the publicationEnvironment International, 2007 also suggests that theShort term toxicity to test was carried out for 48 H to study the effects of Sulfadimethoxine on aquatic invertebrate.

Immobilazation was measured during the test. The Effective concentration EC50 to 50% of Daphnia magna when exposed to Sulfadimethoxine for 48 hr is 248.0 mg/L. It can be concluded from the value that the Sulfadimethoxine is not toxioc to the aquatic invertebrate. and can be considered as "not classified" as per the classification for aquatic environment.

Thus based on the effect concentrations which is in the range 204.963 mg/L to 248.0 mg/L gives the conclusion that test substance 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following three studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to algae was predicted 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2). EC50  Growth value value was estimated to be 109.835 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2) was likely to be not toxic to aquatic algae.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across4-amino-3- methylbenzenesul- fonic acid(CAS: 98 -33 -9) from the ABITEC Lab report, suggests that theFreshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 4-amino-3- methylbenzenesul- fonic acid according to OECD Guideline 201.

The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 0, 30, 45, 67, 100, 150 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance 4-amino-3-methylbenzene- sulfonic acid, in Desmodesmus subspicatus was determined to be 110.6 mg/L

This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP criteria.

Similar supporting experimental study for the structurally similar read across1,3-Phenylenediamine-4-sulfonic Acid(CAS: 88 -63 -1) from the ABITEC Lab report 2017, also suggests that theFreshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 1,3-Phenylenediamine-4-sulfonic Acid according to OECD Guideline 201. The stock solution 150 mg/l was prepared by dissolving light brown powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Sample tested at the concentrations 0, 0, 30, 45, 67, 100 and 150 mg/L. Test performed by the static system for 72hrs of exposure period. Effects on the growth rate of the organism were studied.

The median effective concentration (ErC50) for the test substance 1,3-Phenylenediamine-4-sulfonic Acid, in Desmodesmus subspicatus was determined to be 110 mg/L on the basis of effects on growth rate in a 72 hour study. This value indicates that the substance 1,3-Phenylenediamine-4-sulfonic Acid is likely to be non-hazardous to aquatic algae and cannot be classified as toxic as per the CLP classification criteria.

Thus based on the effect concentrations which is in the range 109.835 mg/L to 110 mg/L gives the conclusion that test substance 2-chlorobenzene-1,4-diammonium sulphate (CAS:  6219-71-2) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.