Registration Dossier

Administrative data

Description of key information

Acute Oral Toxicity: 

In Acute oral toxicity ,LD50 value was predicted based on OECD QSAR toolbox for target substance 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) was estimated to be 3976.64 mg/kg bw,and for differentstudies available on the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5) was considered to be 5000 mg/kg bw and for 1,3-Phenylenediamine-4-sulfonic acid (88-63-1) was considered to be 3480 mg/kg bw. All these studies concluded that the LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute oral toxicity.

Acute Inhalation Toxicity: 

2-chlorobenzene-1,4-diammonium sulphate has very low vapor pressure (2.12E-012 Pa), so the potential for the generation of inhalable vapours is very low. Also the normal conditions of use of this substance will not result in aerosols, particles or droplets of an inhalable size, so exposure to humans via the inhalatory route will be highly unlikely and therefore this end point for acute inhalation toxicity was considered for waiver.

Acute Dermal Toxicity:

In Acute dermal toxicity, LD50 value was predicted based on OECD QSAR toolbox for target substance 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) was estimated to be 20718.78 mg/kg bw andfor differentstudies available on structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5)was considered to be >2000 mg/kg bw and for functionally similar read across substance 4,4'-Diaminodiphenyl sulfone (80-08-0) was considered to be >4000 mg/kg bw.All these studies concluded that the LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute dermal toxicity.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records
Reference
Endpoint:
acute toxicity: oral
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
Justification for type of information:
Data is predicted using OECD QSAR toolbox version 3.3 and QMRF report has been attached
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Prediction was done by using OECD QSAR toolbox v3.3,2017
GLP compliance:
not specified
Test type:
other: estimated data
Limit test:
no
Specific details on test material used for the study:
IUPAC name: 2-chlorobenzene-1,4-diammonium sulphate
Molecular formula: C6H7ClN2.xH2O4S
Molecular weight:240.6661
substance type: organic
physical state: light grey colored solid powder.
InChI:1S/C6H7ClN2.H2O4S/c7-5-3-4(8)1-2-6(5)9;1-5(2,3)4/h1-3H,8-9H2;(H2,1,2,3,4)
Smiles:S(=O)(=O)(O)O.c1(c(ccc(c1)N)N)Cl
Species:
rat
Strain:
other: Gassner
Sex:
male/female
Details on test animals and environmental conditions:
No data available
Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Details on oral exposure:
No data available
Doses:
3976.64 mg/kg bw
No. of animals per sex per dose:
No data available
Control animals:
not specified
Details on study design:
No data available
Statistics:
No data available
Preliminary study:
No data available
Sex:
male/female
Dose descriptor:
LD50
Effect level:
3 976.64 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50% mortality was observed
Mortality:
No data available
Clinical signs:
No data available
Body weight:
No data available
Gross pathology:
No data available
Other findings:
No data available

The prediction was based on dataset comprised from the following descriptors: LD50
Estimation method: Takes average value from the 5 nearest neighbours
Domain  logical expression:Result: In Domain

(((((((("a" or "b" or "c" or "d" or "e" )  and "f" )  and "g" )  and ("h" and ( not "i") )  )  and "j" )  and ("k" and ( not "l") )  )  and ("m" and ( not "n") )  )  and ("o" and "p" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Anilines (Acute toxicity) by US-EPA New Chemical Categories

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Radical OR Radical >> Radical mechanism via ROS formation (indirect) OR Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines OR SN1 OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.3 ONLY

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as SN1 OR SN1 >> Nitrenium Ion formation OR SN1 >> Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD ONLY

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Weak binder, NH2 group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Anilines (amino-para) OR Anilines (Unhindered) OR Inorganic Compound by Aquatic toxicity classification by ECOSAR ONLY

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as No alert found AND Radical AND Radical >> Radical mechanism via ROS formation (indirect) AND Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines AND SN1 AND SN1 >> Nucleophilic attack after metabolic nitrenium ion formation AND SN1 >> Nucleophilic attack after metabolic nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.3 ONLY

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as No alert found AND SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD ONLY

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as Non binder, non cyclic structure AND Weak binder, NH2 group by Estrogen Receptor Binding

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Moderate binder, NH2 group OR Non binder, impaired OH or NH2 group OR Non binder, MW>500 OR Non binder, without OH or NH2 group OR Strong binder, NH2 group OR Very strong binder, OH group OR Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as Reactive unspecified by Acute aquatic toxicity MOA by OASIS ONLY

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as Group 14 - Carbon C AND Group 15 - Nitrogen N AND Group 16 - Oxygen O AND Group 16 - Sulfur S AND Group 17 - Halogens Cl AND Group 17 - Halogens F,Cl,Br,I,At by Chemical elements

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as Group 15 - Phosphorus P OR Group 17 - Halogens Br OR Group 17 - Halogens F by Chemical elements

Domain logical expression index: "m"

Referential boundary: The target chemical should be classified as Aminoaniline, para AND Aniline AND Aryl AND Aryl halide AND No functional group found AND Precursors quinoid compounds by Organic Functional groups

Domain logical expression index: "n"

Referential boundary: The target chemical should be classified as Alkyl arenes OR Amidine OR Aminoaniline, ortho OR Benzimidazole OR Carboxylic acid OR Fused carbocyclic aromatic OR Unsaturated heterocyclic amine OR Unsaturated heterocyclic fragment by Organic Functional groups

Domain logical expression index: "o"

Parametric boundary:The target chemical should have a value of log Kow which is >= -2.2

Domain logical expression index: "p"

Parametric boundary:The target chemical should have a value of log Kow which is <= 0.483

Interpretation of results:
other: not classified
Conclusions:
The LD50 was estimated to be 3976.64 mg/kg bw,when male and female Gassner rats were orally exposed with 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) via gavage.
Executive summary:

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute oral toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) .The LD50 was estimated to be 3976.64 mg/kg bw,when male and female Gassner rats were orally exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) via gavage.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
3 976.64 mg/kg bw
Quality of whole database:
Data is Klimisch 2 and from QSAR toolbox 3.3

Acute toxicity: via inhalation route

Link to relevant study records
Reference
Endpoint:
acute toxicity: inhalation
Data waiving:
other justification
Justification for data waiving:
other:
Endpoint conclusion
Quality of whole database:
waiver

Acute toxicity: via dermal route

Link to relevant study records
Reference
Endpoint:
acute toxicity: dermal
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
Justification for type of information:
Data is predicted using OECD QSAR toolbox version 3.3 and QMRF report has been attached
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Prediction was done by using OECD QSAR toolbox v3.3,2017
GLP compliance:
not specified
Test type:
other: estimated data
Limit test:
no
Specific details on test material used for the study:
IUPAC name: 2-chlorobenzene-1,4-diammonium sulphate
Molecular formula: C6H7ClN2.xH2O4S
Molecular weight:240.6661
substance type: organic
physical state: light grey colored solid powder.
InChI:1S/C6H7ClN2.H2O4S/c7-5-3-4(8)1-2-6(5)9;1-5(2,3)4/h1-3H,8-9H2;(H2,1,2,3,4)
Smiles:S(=O)(=O)(O)O.c1(c(ccc(c1)N)N)Cl
Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals and environmental conditions:
No data available
Type of coverage:
semiocclusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
No data available
Duration of exposure:
24 hours
Doses:
20718.78 mg/kg bw
No. of animals per sex per dose:
No data available
Control animals:
not specified
Details on study design:
No data available
Statistics:
No data available
Preliminary study:
No data available
Sex:
male/female
Dose descriptor:
LD50
Effect level:
20 718.78 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50% Mortality was observed
Mortality:
No data available
Clinical signs:
No data available
Body weight:
No data available
Gross pathology:
No data available
Other findings:
No data available

The prediction was based on dataset comprised from the following descriptors: LD50
Estimation method: Takes average value from the 6 nearest neighbours
Domain  logical expression:Result: In Domain

((((((((("a" or "b" or "c" or "d" or "e" )  and "f" )  and ("g" and ( not "h") )  )  and ("i" and ( not "j") )  )  and ("k" and ( not "l") )  )  and ("m" and ( not "n") )  )  and ("o" and ( not "p") )  )  and "q" )  and ("r" and "s" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Anilines (Acute toxicity) by US-EPA New Chemical Categories

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Radical OR Radical >> Radical mechanism via ROS formation (indirect) OR Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines OR SN1 OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.3 ONLY

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as SN1 OR SN1 >> Nitrenium Ion formation OR SN1 >> Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD ONLY

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Weak binder, NH2 group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Anilines (amino-para) OR Anilines (Unhindered) OR Inorganic Compound by Aquatic toxicity classification by ECOSAR ONLY

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as High (Class III) AND Low (Class I) by Toxic hazard classification by Cramer (with extensions) ONLY

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Aromatic amines AND Inclusion rules not met by Skin irritation/corrosion Inclusion rules by BfR

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as Primary and secondary aliphatic amines by Skin irritation/corrosion Inclusion rules by BfR

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg AND (!Undefined)Group CNHal Lipid Solubility < 400 g/kg AND Exclusion rules not met by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as Group All Melting Point > 200 C OR Group CNHal Aqueous Solubility < 0.004 g/L OR Group CNHal Aqueous Solubility < 0.1 g/L OR Group CNHal log Kow > 3.8 OR Group CNS log Kow > 1.5 OR Group CNS Melting Point > 50 C by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as Group 14 - Carbon C AND Group 15 - Nitrogen N AND Group 16 - Oxygen O AND Group 16 - Sulfur S AND Group 17 - Halogens Cl AND Group 17 - Halogens F,Cl,Br,I,At by Chemical elements

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as Group 15 - Phosphorus P OR Group 17 - Halogens I by Chemical elements

Domain logical expression index: "m"

Referential boundary: The target chemical should be classified as Aminoaniline, para AND Aniline AND Aryl AND Aryl halide AND No functional group found AND Precursors quinoid compounds by Organic Functional groups

Domain logical expression index: "n"

Referential boundary: The target chemical should be classified as Aminoaniline, ortho by Organic Functional groups

Domain logical expression index: "o"

Referential boundary: The target chemical should be classified as Aryl halide AND No functional group found AND Overlapping groups AND Precursors quinoid compounds by Organic Functional groups (nested)

Domain logical expression index: "p"

Referential boundary: The target chemical should be classified as Alkyl arenes OR Aniline OR Nitrobenzene by Organic Functional groups (nested)

Domain logical expression index: "q"

Similarity boundary:Target: Nc1ccc(N)cc1Cl_OS(O)(=O)=O
Threshold=20%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "r"

Parametric boundary:The target chemical should have a value of log Kow which is >= -1.23

Domain logical expression index: "s"

Parametric boundary:The target chemical should have a value of log Kow which is <= 0.241

Interpretation of results:
other: not classified
Conclusions:
The LD50 value was estimated to be 20718.78 mg/kg bw,when male and female New Zealand White rabbits were semiocclusively exposed with 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) by dermal application for 24 hours.
Executive summary:

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute dermal toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2).The LD50 was estimated to be 20718.78 mg/kg bw,when male and female New Zealand White rabbits were semiocclusively exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2)by dermal application for 24 hours.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
20 718.78 mg/kg bw
Quality of whole database:
Data is Klimisch 2 and from QSAR toolbox 3.3

Additional information

Acute Oral Toxicity: 

In different studies, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) has been investigated for acute oral toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments and estimated data in rodents, i.e. most commonly in rats for 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) along with the study available on the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5) and 1,3-Phenylenediamine-4-sulfonic acid (88-63-1). The predicted data using the OECD QSAR toolbox has also been compared with the experimental studies. The studies are summarized as below –

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute oral toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2).The LD50 was estimated to be 3976.64 mg/kg bw,when male and female Gassner rats were orally exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) via gavage.

The above experimental study was supported by Sustainability Support Services (Europe) AB (study number: 19166, 2017-08-10) for the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5). The acute oral toxicity profile of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5)in Sprague Dawley rats. Initially, three female animals were treated at the dose level of 300 mg/kg body weight of the test item (Step - I). Administration of the test item at 300 mg/kg did not result in any signs of toxicity and mortality at 24 hours after the dosing. As no mortality was observed at 24 hours after the dosing, three female animals were added to the study and treated with the same dose of 300 mg/kg of the test item (Step - II). Administration of the test item at 300 mg/kg did not result in any signs of toxicity and mortality after the dosing. No mortality was observed at 300 mg/kg dose group, hence additional three female animals were treated with the higher dose of 2000 mg/kg of the test item (Step - I). Administration of the test item at 2000 mg/kg did not result in any signs of toxicity and mortality after the dosing. As no mortality were observed at 24 hours after the dosing, additional three female animals were treated with the higher dose of  2000 mg/kg of the test item (Step - II). Administration of the test item at 2000 mg/kg did not result in any signs of toxicity and mortality after the dosing.Gross pathological examination did not reveal any abnormalities in animals from 300 mg/kg and 2000 mg/kg dose groups. The acute oral LD50 (Cut-off value) of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5) was 5000 mg/kg body weight. Thus, it was concluded that the acute toxicity study of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5), when administered via oral route in Sprague Dawley rats falls into the “Category Unclassified” criteria of CLP.

This is further supported by Richard J Lewis (Sax’s Dangerous Properties of Industrial Materials, 12th Edition); IFA GESTIS (Gestis Substance Database,2017) and U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the structurally similar read across substance 1,3-Phenylenediamine-4-sulfonic acid (88-63-1).Acute oral toxicity test of 1,3-Phenylenediamine-4-sulfonic acid was conducted with rat in the concentration of 3480 mg/kg bw orally. 50% mortality was observed in treated rat. Therefore, LD50 was considered to be 3480 mg/kg, when rats were treated with 2,4-diaminobenzenesulphonic acid orally.

Thus, based on the above studies on 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) and it’s structurally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) cannot be classified for acute oral toxicity.

Acute Inhalation Toxicity: 

2-chlorobenzene-1,4-diammonium sulphate has very low vapor pressure (2.12E-012 Pa), so the potential for the generation of inhalable vapours is very low. Also the normal conditions of use of this substance will not result in aerosols, particles or droplets of an inhalable size, so exposure to humans via the inhalatory route will be highly unlikely and therefore this end point for acute inhalation toxicity was considered for waiver.

Acute Dermal Toxicity:

In different studies, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) has been investigated for acute dermal toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments and estimated data in rodents, i.e. most commonly in rabbits for 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) along with the study available on structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5)and on functionally similar read across substance 4,4'-Diaminodiphenyl sulfone (80-08-0). The predicted data using the OECD QSAR toolbox has also been compared with the experimental studies. The studies are summarized as below –

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute dermal toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2).The LD50 was estimated to be 20718.78 mg/kg bw,when male and female New Zealand White rabbits were semiocclusively exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2)by dermal application for 24 hours.

The above study was further supported by Sustainability Support Services (Europe) AB (study number: 19167, 2017-08-10) for the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5).The acute dermal toxicity profile of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5)in Sprague Dawley rats.The test item was applied to shorn skin of 5 male and 5 female animals at 2000 mg/kg body weight. Administration of the test item at 2000 mg/kg did not result in any skin reaction at the site of application during the study period of 14 days. Administration of the test item did not result in any signs of toxicity and mortality during the study period of 14 days.Animals exhibited normal body weight gain through the study period of 14 days.Gross pathological examination did not reveal any abnormalities attributable to the treatment. It was concluded that the acute dermal median lethal dose (LD50) of 6-aminonaphthalene-2-sulphonic acid (93-00-5), when administered to male and female Sprague Dawley rats was considered to be >2000 mg/kg body weight.Thus, according to CLP criteria for acute toxicity rating for the chemicals, it infers that 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5) does not classify as an acute dermal toxicant.

Also these results are further supported by CHARLES P. CARPENTER et. al. (TOXICOLOGY AND APPLIED PHARMACOLOGY 28,313-319 (1974)); U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) and IFA GESTIS (Gestis Substance Database,2017) for the functionally similar read substance4,4'-Diaminodiphenyl sulfone (80-08-0).In acute dermal toxicity study,rabbits were treated with 4,4'-Diaminodiphenyl sulfone (80-08-0)in the concentration of 4000 mg/kg bw by dermal application.No mortality was observed in treated rabbits at dose 4000 mg/kg bw.Therefore, LD50 value was considered to be >4000 mg/kg bw,when rabbits were treated with 4,4'-Diaminodiphenyl sulfone (80-08-0) by dermal application.

Thus, based on the above studies on 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) and it’s structurally and functionally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute dermal toxicity.

Justification for classification or non-classification

Based on the above studies and prediction on 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) and it’s structurally and functionally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute oral and dermal toxicity. For Acute inhalation toxicity wavier was added so, not possible to classify.