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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF

HOUSING CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: ca. 5 µE/m²s at a wavelength of 400-750 nm

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.78 mmol/L
Test temperature:
ca. 21 °C
pH:
7.9 - 8.2
Dissolved oxygen:
Study initiation: 9.00 - 9.25 mg/L
Study end: 8.51 - 8.76 mg/L
Nominal and measured concentrations:
Nominal test concentrations: control, 62.5, 125, 250, 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, fill volume: centrifuge tubes with flat bottom, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water (conductivity < 0.05 µS/cm) aerated with oil-free air for 24 h
- Alkalinity: 0.92 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 660 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen content after 0 and 48 hours

OTHER TEST CONDITIONS
- Light intensity: diffuse light

EFFECT PARAMETERS MEASURED: mobility after 0, 3, 6, 24, 48 hours

Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Details on results:
- Mortality of control: none

Description of key information

With high probability sodium prop-2-enesulphonate is acutely not harmful to aquatic invertebrates.

Key value for chemical safety assessment

Additional information

The acute toxicity of sodium prop-2-enesulphonate was studied with the water flea Daphnia magna, in accordance to EU Method C.2 [BASF AG, 1989]. Up to the highest test concentration of 500 mg/L sodium prop-2 -enesulphonate no effect observed.