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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with analytical monitoring
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Separately for each concentration 0.5007, 1.0012, 10.0, 21.5, 46.4 and 100.0 g test substance were added to the test water using a glass stick. Subsequently the fish were placed into the aquaria within 15 min after the test material was added.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation: about 4 month
- Length at study initiation: 2.9 cm (2.7 - 3.1 cm)
- Weight at study initiation: 0.233 g (0.17 - 0.3 g)
- Feeding during test
- Food type: Standard feed (TETRA MIN), on workdays generally additionally live artemia
- Amount: ad libitum


ACCLIMATION
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): 0% mortality during the last 14 days prior to test initiation
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
Total hardness: about 2.5 mmol/L (about 250 mg/L CaCO3)
Test temperature:
22 - 23 °C
pH:
8.3 - 8.7
Dissolved oxygen:
7.7 - 8.4 mg/L
Nominal and measured concentrations:
Nominal test concentration: control, 50.0, 100.0, 1000.0, 2150.0, 4640.0, 10000.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: all-glass aquaria, 30 x 22 x 24 cm, 10 L fill volume (water depth about 17 cm)
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.23 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated, char coal filtered
- Ca/Mg ratio: 9:1
- Intervals of water quality measurement: after 1, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness

EFFECT PARAMETERS MEASURED: mortality and symptoms after 1, 4, 24, 48, 72, 96 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approximately 2.2
- Range finding study: yes
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 4 600 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
2 150 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 4 500 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
2 050 mg/L
Nominal / measured:
meas. (not specified)
Details on results:
- Behavioural abnormalities: none
- Mortality of control: none

Description of key information

With high probability sodium prop-2-enesulphonate is acutely not harmful to fish.

Key value for chemical safety assessment

Additional information

According to OECD guideline 203 a toxicity test with the Zebra fish Danio rerio was conducted [BASF AG, 1994]. The measured LC50 (96h) value was approx. 4500 mg/L and the NOEC was 2050 mg/L sodium prop-2-enesulphonate.