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EC number: 248-654-8 | CAS number: 27776-01-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- For the purpose of chemical analysis during the definitive test, duplicate aliquots (ca 20 mL) were removed from test and control solutions at the commencement of the test as 0 h samples. At test termination, an aliquot (ca 20 mL) was removed from each replicate flask and combined separately, for the test and control solutions. Duplicate aliquots (ca 20 mL) were removed from the combined solutions as 72 h samples.
An additional flask was prepared at nominal loading rates of 4 and 0.25 mg benzyltoluene/L and these incubated (without algal cells) with the test and control flasks. At 72 h, duplicate aliquots (ca 20 mL) were removed from these additional flasks for analysis, to determine any differences in measured concentrations of benzyltoluene between cell-free medium and medium containing cells. - Vehicle:
- no
- Details on test solutions:
- Individual WAF’s were prepared by adding weighed amounts of benzyltoluene (5.9, 11.6, 23.0, 46.0 and 92.1 mg) to algal medium (23 litre) and stirring these in the dark for ca 25 h. A control WAF (algal medium only) was prepared in an identical manner to that of the test WAF’s. The rate of stirring was adjusted to ensure the vortex was =1% of the depth of the water column. Following the period of stirring, the contents were allowed to settle for ca 25 h, after which ca 1100 mL of solution was siphoned from the centre of the vessels, with the first ca 100 mL being discarded. The remaining ca 1000 mL of solution was used as the test and control solutions and as samples for chemical analysis.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The freshwater green alga Pseudokirchneriella subcapitata CCAP 278/4 was used for this study. Starter cultures were obtained from CCAP Oban, UK on 14 July 2009 (limit test) and 09 March 2010 (definitive test).
Transfers of P. subcapitata were made regularly into fresh growth medium to provide suitable subcultures, in the exponential phase of growth, for test inoculations. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- All testing and algal culturing was conducted using ISO freshwater algal medium
- Test temperature:
- 23.0- 23.4 °C
- pH:
- Initial 7.53
Final 8.44 - 8.96 - Dissolved oxygen:
- Not a parameter to be monitored in application of the Guideline
- Salinity:
- All testing and algal culturing was conducted using ISO freshwater algal medium
- Nominal and measured concentrations:
- Test solutions were prepared through the WAF method.
Loading rates were: 0 - 0.25 - 0.5 - 1 - 2 and 4 mg/L
Measured Initial concentrations were 0 - 212 - 281 - 306 - 414 - 505 µg/L
All final (72 h) measured concentrations were below limit of quantification (61 µg/L)
Concentrations used for calculation were geometric mean between initial and loq concentrations: 0 - 114 - 131 - 137 - 159 and 176 µg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: erlenmeyer flasks
- Material, size, headspace, fill volume: glass, 250 mL capacity, 100 mL medium volume
- Aeration: capped with foil lids
- Initial cells density:0.86 e+4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, according to ISO, as recoomended in OECD 201
OTHER TEST CONDITIONS
- Sterile test conditions: no
- adjustement of pH: no
- Photoperiod: continuous
- Light intensity and quality: uniform cool white, 8067 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: limit test with WAF loading rate 5 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 176 µg/L
- Basis for effect:
- growth rate
- Remarks on result:
- other: the maximum achievable solubility under the conditions of the test.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 159 µg/L
- Basis for effect:
- growth rate
- Remarks on result:
- other: the maximum achievable solubility under the conditions of the test.
- Details on results:
- The following results are based on geometric mean measured concentrations of benzyltoluene:
From growth rate data, the EC10 at 24 h was estimated as 147 µg/L with 95% confidence limits of 128 and 169 µg/L. Due to the lack of growth inhibition, no other EC values could be estimated at any time point. It is concluded that benzyltoluene does not affect algal growth rate over a 72 h period, at the maximum achievable solubility under the conditions of the test.
The pH of test solutions and the temperature of the environmental area were maintained within protocol specification throughout the test. The average light intensity measured at flask level during the definitive test was 8067 lux. - Reported statistics and error estimates:
- Growth rates were analysed for homogeneity of variance using Levene's test at a 1% significance level. Following ANOVA, benzyltoluene treated groups were compared to the control using a one-sided Dunnett's test (tested for a decrease) at a 5% significance level. For regression analysis, a probit transformation was applied followed by regression against logarithmetically transformed concentrations of test material with a Newton-Raphson maximum likelihood iterative procedure being used to obtain parameter estimates. From the fitted model, the ECx value was estimated.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72 h EC50 value for average specific growth rate and yield was estimated to be greater than the previous maximum solubility of benzyltoluene under the conditions of the test. It is concluded that benzyltoluene does not affect algal growth rate over a 72 h period, at the maximum achievable solubility under the conditions of the test.
- Executive summary:
This study was designed to determine the effects of benzyltoluene on the growth of Pseudokirchneriella subcapitata, a unicellular freshwater green alga, over a 72 h period. The study was performed to meet the requirements of OECD (2006) Guideline 201.
Based on the outcome of a limit test, a definitive test was conducted with test solutions prepared as water accommodated fractions (WAF) from initial loading rates of 0.25, 0.5, 1, 2 and 4 mg benzyltoluene/L. A control of untreated algal medium was included in the test. The method of preparation was selected to ensure the test item was tested at the maximum achievable solubility, under the conditions of the test.
Analytical samples taken at 0 h indicated that benzyltoluene is only partially soluble in algal medium, with concentrations ranging from 212 µg/L to 505 µg/L. Measured concentrations at 72 h indicated that the initial concentrations of benzyltoluene were not maintained. The loss over the 72 h period was ca100% with all test concentrations being below the limit of detection (limit of quantification = 61 µg/L). The overall (0 and 72 h) geometric mean measured concentrations of benzyltoluene ranged from 114 µg/L to 176 µg/L.
The 72 h EC50 and 72h EC10 values for average specific growth rate and yield were estimated to be greater than the maximum solubility of benzyltoluene under the conditions of the test. From the growth rate results of this study, it was not possible to derive a LOEC and thus it was not relevant to estimate a NOEC.
Therefore, it is concluded that benzyltoluene does not affect algal growth rate over a 72 h period, at the maximum achievable solubility under the conditions of the test.
Reference
Geometric mean measured concentration of benzyltoluene (µg/L) |
Replicate flask |
Mean cell concentrations (x105cells/mL) |
|||
0 h (inoculum) |
24 h |
48 h |
72 h |
||
0 (control) |
1 |
0.09 |
0.65 |
4.35 |
15.55 |
2 |
0.09 |
0.58 |
4.90 |
15.28 |
|
3 |
0.09 |
0.65 |
3.68 |
13.25 |
|
4 |
0.09 |
0.65 |
4.00 |
15.53 |
|
5 |
0.09 |
0.43 |
3.83 |
15.18 |
|
6 |
0.09 |
0.85 |
3.88 |
15.85 |
|
114 |
1 |
0.09 |
0.63 |
2.45 |
10.90 |
2 |
0.09 |
0.48 |
1.80 |
8.98 |
|
3 |
0.09 |
0.50 |
4.03 |
12.53 |
|
131 |
1 |
0.09 |
0.60 |
3.58 |
15.35 |
2 |
0.09 |
0.58 |
4.53 |
16.70 |
|
3 |
0.09 |
0.55 |
4.08 |
15.93 |
|
137 |
1 |
0.09 |
0.63 |
2.30 |
10.40 |
2 |
0.09 |
0.55 |
2.68 |
12.80 |
|
3 |
0.09 |
0.45 |
1.83 |
9.40 |
|
159 |
1 |
0.09 |
0.48 |
2.13 |
9.60 |
2 |
0.09 |
0.58 |
3.68 |
14.73 |
|
3 |
0.09 |
0.53 |
4.00 |
16.03 |
|
176 |
1 |
0.09 |
0.38 |
1.80 |
8.85 |
2 |
0.09 |
0.60 |
3.23 |
11.83 |
|
3 |
0.09 |
0.33 |
3.33 |
12.68 |
Table 1: Cell concentration per replicate per sampling time.
Geometric mean measured concentration of benzyltoluene (µg/L) |
Time (h) |
Measured concentrations of benzyltoluene (µg/L) |
Geometric mean measured concentrations of benzyltoluene (µg/L) |
Control |
0 |
< LOD |
0 |
72 |
< LOD |
||
114 |
0 |
212 |
114 |
72 |
< LOD |
||
131 |
0 |
281 |
131 |
72 |
< LOD |
||
137 |
0 |
306 |
137 |
72 |
< LOD |
||
159 |
0 |
414 |
159 |
72 |
< LOD |
||
176 |
0 |
505 |
176 |
72 |
< LOD |
Table 2:Measured concentrations of benzyltoluene in test solutions during the test. LOD = 61 µg/L
Geometric mean measured concentration of benzyltoluene (µg/L) |
Replicate flask |
Mean cell concentrations (x105cells/mL) |
|
0 h |
72 h |
||
0 (control) |
1 |
7.53 |
8.84 |
2 |
8.93 |
||
3 |
8.90 |
||
4 |
8.91 |
||
5 |
8.93 |
||
6 |
8.81 |
||
114 |
1 |
7.53 |
8.73 |
2 |
8.44 |
||
3 |
8.92 |
||
131 |
1 |
7.52 |
8.96 |
2 |
8.98 |
||
3 |
8.87 |
||
137 |
1 |
7.53 |
8.90 |
2 |
8.93 |
||
3 |
8.62 |
||
159 |
1 |
7.53 |
8.88 |
2 |
8.82 |
||
3 |
8.95 |
||
176 |
1 |
7.51 |
8.67 |
2 |
8.92 |
||
3 |
8.93 |
Table 3: pH evolution from 0h to 72h in each replicate during the test.
Description of key information
In an OECD 201 TG compliant study performed under GLP, benzyltoluene did not affect algal growth rate over a 72 h period at the maximum achievable solubility.
Key value for chemical safety assessment
Additional information
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