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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 1967
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1967
Report date:
1967

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
yes
Remarks:
50 µl test substance, no wash-out, shorter observation period
Principles of method if other than guideline:
Before OECD Guideline 405 was established, eye irritation was tested using an internal method (BASF test).
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
But-3-yn-2-ol
EC Number:
217-978-1
EC Name:
But-3-yn-2-ol
Cas Number:
2028-63-9
Molecular formula:
C4H6O
IUPAC Name:
but-3-yn-2-ol
Details on test material:
purity 56.1%
other component: water
according to order sheet from Dec 2nd 1988

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
No further details provided

Test system

Vehicle:
water
Controls:
other: 0.9 % NaCl solution in other eye of the same animal
Amount / concentration applied:
0.05 mL of 55 % test substance was applied.
Duration of treatment / exposure:
The test substance was applied once and not removed from the eye by washing.
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
other: 1 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
other: 1 h
Score:
1
Max. score:
3
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
other: 1 h
Score:
2
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
other: 1 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
other: 1 h
Score:
1
Max. score:
3
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
other: 1 h
Score:
2
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
other: 24 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
other: 24 h
Score:
1
Max. score:
3
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
other: 24 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
other: 24 h
Score:
2
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
other: 24 h
Score:
2
Max. score:
3
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
other: 24 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Other effects:
Pathology showed a slight hydrothorax, starting lungedema and diarrhea.
After 8 days in both animals scar formation on the treated eye was observed.
The report usually describes findings after 1 and 24 hours, and at the end of the observation period. Thus, for a final evaluation, the findings after 48 and 72 hours from the raw data have to be taken into account.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria