But-3-yn-2-ol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 1967

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

exposure for 1, 5, 15 min, 20 h, 24 h; shorter observaton period

Principles of method if other than guideline:

Before OECD Guideline 404 was established in 1982, skin irritation was tested using an internal method (BASF test).

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

But-3-yn-2-ol

EC Number:

217-978-1

EC Name:

But-3-yn-2-ol

Cas Number:

2028-63-9

Molecular formula:

C4H6O

IUPAC Name:

but-3-yn-2-ol

Details on test material:

purity 56.1%
other component: water
according to order sheet from Dec 2nd 1988

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

No further details were provided

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

water

Controls:

no

Amount / concentration applied:

The test substance was applied in a 55 % solution in water. 0.2 mL/kg bw were used.

Duration of treatment / exposure:

2 animals were treated on different skin areas for 1, 5 and 15 min;
2 animals were treated for 20 h.

Observation period:

8 days

Number of animals:

4

Details on study design:

After the 1, 5 and 15 min or 20 h application the test substance was washed off. First it was washed with Lutol 9 (100 %) and afterwards with Lutrol 9 in water (50 %). Animals were fixated by binding of the animal to an exposure board during application.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

other: 3.5 h

Max. score:

4

Reversibility:

not reversible

Remarks on result:

other: animal died

Results 2

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 3.5 h

Max. score:

4

Remarks on result:

other: animal died

Other effects:

Treatment for 20 h led to the death of the animals during exposure. Animals died 3.5 to 20 h after application.
Pathology revealed an increased fluid accumulation in the thoracic region, starting lungedema and diarrhea.

Any other information on results incl. tables

		24h		48 h		d7
exposure	animal #1	erythema	edema	erythema	edema	erythema	edema
1 ´	#1	-	-	-	-	-	-
	#2	2	-	1	-	-	-
5´	#1	-	-	-	-	-	-
	#2	1	-	1	-	-	-
15´	#1	-	-	-	-	-	-
	#2	1	-	1	-	-	-
20 h	#1	exitus over night
	#2	exitus over night

OECD / Draize Score (max: 4)

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria