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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 1957
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1958
Report date:
1958

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
lower animal number/group, only 2 doses tested
Principles of method if other than guideline:
according to Hill ("The manufacturing chemist's Ass. Program for the labeling of hazardouos chemicals"; Ass. Food. and Drug Off. 18, 142, 1954).
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
But-3-yn-2-ol
EC Number:
217-978-1
EC Name:
But-3-yn-2-ol
Cas Number:
2028-63-9
Molecular formula:
C4H6O
IUPAC Name:
but-3-yn-2-ol
Specific details on test material used for the study:
The pure substance and a solution of 40% were tested.

Test animals

Species:
rabbit
Strain:
Vienna White
Sex:
not specified
Details on test animals or test system and environmental conditions:
No data provided

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: water and unchanged
Details on dermal exposure:
TEST SITE
- Area of exposure: back skin, shaved, 50 cm2
- Type of wrap if used: lamination

REMOVAL OF TEST SUBSTANCE
Test substance was removed by removing the wrap.

TEST MATERIAL
- Amount(s) applied: 200 mm3/kg (pure) and 80mm3/kg (40 %)
- Concentration: pure and 40 % in water
- Constant volume or concentration used: yes

VEHICLE
For 40 % test substance was mixed with water.
Duration of exposure:
24 hours
Doses:
80 and 200 mm3/kg
69 and 172 mg/kg bw (calculated based on density at 20 °C = 0.8616 g/ml, CRC 2008)
No. of animals per sex per dose:
3 animals per dose but no sex distribution provided
Control animals:
no
Details on study design:
- Duration of observation period following administration: 4 weeks
- Frequency of observations and weighing: every 1 - 3 days
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight, histopathology
Statistics:
No statistical analysis was performed.

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 69 - < 172 mg/kg bw
Based on:
test mat.
Remarks on result:
other: original range: > 80 to < 200 mm3/kg bw , mg/kg bw calculated based on density at 20 °C = 0.8616 g/ml
Mortality:
All animals treated with the pure test substance (200 mm3/kg bw) died within 8 - 24 hours after application.
All animals treated with the 40 % test substance (80 mm3/kg bw) survived the application.
Clinical signs:
Animals treated with the pure test substance developed apathy and diarrhea before death.
Animals treated with the 40 % test substance were atonic and apathetic but recovered within 24 h.
Body weight:
Survivors showed no weight loss.
Gross pathology:
Increased fluid accumulation in the thoracic region, liver and kidneys enrichment with blood was detected in animals treated with the pure test substance.

Applicant's summary and conclusion

Interpretation of results:
Category 2 based on GHS criteria