Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 256-181-3 | CAS number: 45021-77-0
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
- Report date:
- 1975
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- Pre-GLP
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Diallyldimethylammonium chloride
- EC Number:
- 230-993-8
- EC Name:
- Diallyldimethylammonium chloride
- Cas Number:
- 7398-69-8
- IUPAC Name:
- N-allyl-N,N-dimethylprop-2-en-1-aminium chloride
- Details on test material:
- - Name of test material (as cited in study report): Diallydimethylammonium chloride (64.3% solution in water).
- Molecular formula (if other than submission substance): C8H16N.Cl
- Molecular weight (if other than submission substance): 161.68
- Smiles notation (if other than submission substance): C(=C)CN{+}(c)(c)(.Cl{-})CC=C
- InChl (if other than submission substance): 1/C8H16N.ClH/c1-5-7-9(3,4)8-6-2;/h5-6H,1-2,7-8H2,3-4H3;1H/q+1;/p-1
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Paderborn, Germany
- Age at study initiation: unknown
- Weight at study initiation: 180 g
- Fasting period before study: 18-20h
- Housing: individual cages
- Diet: ad libitum (Saniff/Intermast)
- Water: ad libitum
- Acclimation period: 4 days
ENVIRONMENTAL CONDITIONS: no data given
IN-LIFE DATES: Unknown
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 0, 3.18, 3.98, 5.00 and 6.30 ml/kg
- No. of animals per sex per dose:
- 5 males/5 females
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: at 15, 30, 60, 120, and 240 minutes after dosing and daily thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, gross pathology - Statistics:
- The LD50 was calculated according to the method of "Thomson and Weil" (Biometrics 8, 51-54, 1952) at 24h and 14 days.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- 3.18 mL/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 2044 mg/kg bw effecrtive dose
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 4.81 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- > 4.41 - <= 5.53
- Remarks on result:
- other: 3093 mg/kg bw effective dose
- Sex:
- male/female
- Dose descriptor:
- LD100
- Effect level:
- 6.3 mL/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 4047 mg/kg bw effective dose
- Mortality:
- Mortality was as follows:
Group Dose (ml/kg) 1 day mortality 14 day mortality
I 3.18 0/10 0/10
II 3.98 1/10 1/10
III 5.00 6/10 6/10
IV 6.30 10/10 10/10
- Clinical signs:
- Piloerection, ataxia, reduce motor activity, body tremor, death.
- Body weight:
- Group Mean body Weight day 0 Mean body Weight day 14
I 176.5 g 202.5 g
II 175.5 g 200.4 g
III 175.0 g 202.5 g
IV 175.0 g not weighed - Gross pathology:
- All animals showed a high hyperemia of the epithelium of the stomach, jejunum and colon.
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The oral LD50 for the 63% solution of DADMAC was calculated as 4.81 (4.41-5.53) ml/kg which is the equivalent of 3093 DADMAC mg/kg bw. The No Observed Adverse Effect Level was 3.18 ml/kg bw, equivalent to 2044 mg DADAMAC/kg bw.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
