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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dimethyldiallylammoniumchloride ca 60%
- Molecular formula (if other than submission substance): C8H16N.Cl
- Molecular weight (if other than submission substance): 161.68
- Smiles notation (if other than submission substance): C(=C)CN{+}(c)(c)(.Cl{-})CC=C
- InChl (if other than submission substance): 1/C8H16N.ClH/c1-5-7-9(3,4)8-6-2;/h5-6H,1-2,7-8H2,3-4H3;1H/q+1;/p-1
- Substance type: non-volatile, organic
- Physical state: colourless liquid
- Composition of test material, percentage of components: 60% aqueous solution of Dimethyldiallylammonium chloride
- Analytical purity: no data given
- Impurities (identity and concentrations): no data given
- Lot/batch No.: 76334
- Expiration date of the lot/batch: no data given
- Stability under test conditions: satable
- Storage condition of test material: room temperture

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge : BASF laboratory wastewater plants treating municipal sewage
- Concentration of sludge: 30mg/L
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
54 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: BASF laboratory wastewater plants treating municipal sewage:
- Test temperature: room temperature
- pH: 7.4-7.7
- pH adjusted: no
- Aeration of dilution water: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 1000 ml vessels
- Number of culture flasks/concentration: 2 for test substance, 1 for the controls and the reference substance
- Method used to create aerobic conditions: no data
- Measuring equipment: no data
- Test performed in open system: no
SAMPLING
- Sampling frequency: Sample were taken for DOC analysis at days 0,1, 3, 5, 7, 10, 14, 17, 22, 24, 27, and 28
- Sampling method: no data
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: Degradation degree in the inhibition control after 14days
- Abiotic sterile control: Physico-chemical (abiotic) elimination of the test substance
- Toxicity control: no data
- Other: Elimination of the test substance by absorption
At day 14 of the test 1ml mercury chloride was added to the abiotic control assay
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
Validity criteria: The test is valid
Deviation of the degradation degree of the test substance in the plateau phase < 20%
Degradation degree of the reference substance > 70% after 14 days.
Degradation degree in the inhibition control > 35% after 14 days
% Degradationopen allclose all
Parameter:
% degradation (DOC removal)
Value:
>= 40 - <= 50
Sampling time:
28 d
Parameter:
% degradation (DOC removal)
Value:
ca. 0
Sampling time:
10 d
Details on results:
Degradation degree in the inhibition control after 14days: 40-50 %DOC
Physico-chemical (abiotic) elimination of the test substance: <10 % DOC
Elimination of the test subtance by absorption: <10 % DOC after 5 days

BOD5 / COD results

Results with reference substance:
Degradation % DOC 14 days = 90-100

Any other information on results incl. tables

DOC Decrease %

 Day  RS IH  PC   AC TS1  TS2   TS Mean value
0 0 0 0 0
1 2 -1 -4 -4  -2  -2  -2
3 25 3 -12 -13  -6  -12  -9
5 94 46 -9 -10  -5  -6  -6
7  98 42 -17   -10  -12  -11 
10 96 45 -9   -4  -9  -7 
14 96 47 -9   -2  -1 
17 93 49 -9   38  28  33 
22 97 73 0   46  45  46 
24 95 71 -9   43  40  42 
 27 99 70 -14   41  43  42 
 28 97 69 -13   45  43  44 

RS = reference substance, IH = inhibition control, PC = abiotic control, AC = absorption control, TS = test substance

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Degradation of the test substance was 40-50% (but less than 70%) measured by DOC removal in 28 days. Consequently dimethyldiallylammonium chloride is not readily biodegradable according to the OECD criteria.