Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dimethyldiallylammoniumchlorid ca.60%
- Molecular formula (if other than submission substance): C8H16N.Cl
- Molecular weight (if other than submission substance): 161.68
- Smiles notation (if other than submission substance): C(=C)CN{+}(c)(c)(.Cl{-})CC=C
- InChl (if other than submission substance): 1/C8H16N.ClH/c1-5-7-9(3,4)8-6-2;/h5-6H,1-2,7-8H2,3-4H3;1H/q+1;/p-1
- Substance type: non-volatile, organic
- Physical state: liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: aqueous solution (at about 60%)
- Lot/batch No.: R 950 Partie 15
- Expiration date of the lot/batch:
- Stability under test conditions: stable
- Storage condition of test material: no data

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0, 6.25 and 100 m/l
- Sampling method: At the start of the test (0h), samples from vessels without daphnids, at the end of the test (after 48h) samples from vessels with and without daphnids.
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:
yes
Details on test solutions:
M4 water (according to ISO 10706)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia
- Strain: Daphnia magna STRAUS 1820
- Source: in house breeding (sourced from Institut National de Recherche Chimique Appliquée, France in 1978)
- Age at study initiation: 2-24 hours (starting with the 3rd breed of parent animals)
- Feeding during test: No

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None

Test conditions

Hardness:
2.20-3.20 mmol/L
Test temperature:
20.3-20.4°C
pH:
8.0-8.2
Dissolved oxygen:
8.5-8.7 mg/L
Salinity:
no
Nominal and measured concentrations:
6.25, 12.5, 25, 50, and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom 20ml
- Type : open
- Material, fill volume: 10 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized water
- Total organic carbon: none
- Particulate matter: none
- Metals: none
- Pesticides: none
- Chlorine: none
- Alkalinity: 0.80-1.00 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: 550-650 µS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: artificial light16 h light/8 h dark
- Light intensity: about 2-7µE/(m2.s) at wave length of 400-700nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Swimming ability observed (visual) after 0, 3, 6 24 and 48 hours)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95%CL
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
DADMAC in aqueous solution at 60% concentration had no effect on the swimming ability of daphnia magna at a test concentration of 50 mg/l. The EC50 and EC 100 were both greater than 100mg/L. Therefore, the EC0 for DADMAC is 30 mg/l and the EC50 and EC100 are both greater than 60 mg/l.