Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16/06/1996-21/06/1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented and scientifically acceptable report.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
yes
Remarks:
The growth characteristics of the bacteria were not measured by turbidimetr by using a microbiological method with Petri dish cultures according to Bringmann and Kühn, 1977.
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material: DIMAPA Quat
- Physical state: liquid
- Analytical purity: 60%
- Composition of test material, percentage of components: 100%
- Lot/batch No.: not specified
- pH-value: 8
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
Demineralised water

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
5 d

Test conditions

Nominal and measured concentrations:
The nominal concentrations tested were 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000, 2000, 4000 mg/L.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
5 d
Dose descriptor:
NOEC
Effect conc.:
4 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
APTAC had no inhibitory effect on the growth of Pseudonomas putida at 4000 mg/l after 5 days.