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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)
Principles of method if other than guideline:
None
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: Seeding water of an urban waste water
Details on inoculum:
Seeding water of an urban waste water
Duration of test (contact time):
5 d
Initial conc.:
>= 6.6 - <= 844.6 mg/L
Based on:
test mat.
Details on study design:
Validity
Blank: BOD5 : should preferably not exceed 0,5 mg O2/I
Reference substance: BOD5 : between 180 - 230 mg O2/I
Test substance: 02-concentration after 5 days : between one-third and two-thirds of the initial 02-concentration
Reference substance:
other: D(+) - Glucose/L-Glutamic acid
Remarks on result:
not measured/tested
Key result
Parameter:
BOD5
Value:
3 mg O2/g test mat.
Results with reference substance:
The BOD5 of the reference substance is : 195,180, 200 mg O2/I.

None

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The BOD of FAT 40000 is: 3.0 mg O2/g test mat.
Executive summary:

The biochemical oxygen demand of FAT 40000/G is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20°C in different test concentrations diluted in water saturated with dissolved oxygen and containing a seed of microorganisms. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 mg/l. The BOD5 of FAT 40000/G: 3 mg 02/g The BOD5 of the reference substance is : 195,180, 200 mg O2/I

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
other: EEC Directive 84/449 (1984), Part. C.9 according to DEV H 41-1, Deutsches Einheits-Verfahren, 1980
Deviations:
yes
Remarks:
Mean of 2 determinations instead of 3
Principles of method if other than guideline:
None
GLP compliance:
yes
Specific details on test material used for the study:
None
Oxygen conditions:
aerobic
Inoculum or test system:
not specified
Details on inoculum:
None
Duration of test (contact time):
2 h
Initial conc.:
5 mg/L
Based on:
COD
Details on study design:
None
Reference substance:
other: Potassium hydrogen phthalate solution
Remarks on result:
not measured/tested
Key result
Parameter:
COD
Value:
402 mg O2/g test mat.
Results with reference substance:
The COD of the reference substance is : 193 mg O2/I

None

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The COD of the test substance FAT 40000 is: 402 mg 02/g.
Executive summary:

A predeterminated amount of the test substance FAT 40000/G dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate (according to DEV H 41-1, DEV1980). The test substance concentration was tested in 5.0 mg/l. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. The COD of FAT 40000/G is : 402 mg 02/g The COD of the reference substance is: 193 mg O2/I

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Principles of method if other than guideline:
None
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: Sludge of a biological sewage treatment plant
Details on inoculum:
Sludge of a biological sewage treatment plant (ARA-Basel Industries) collected on 16.02.93
Duration of test (contact time):
28 d
Initial conc.:
150 mg/L
Based on:
DOC
Details on study design:
None
Reference substance:
diethylene glycol
Preliminary study:
None
Test performance:
None
Key result
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Remarks on result:
other: Standard deviation not available
Details on results:
None
Results with reference substance:
The bioelimination of the reference substance after 16 days is : 95.2 %.

None

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Biodegradation of the test substance after 28 days was 0 %.
Executive summary:

A key study was performed to determine the percent biodegradation of FAT 40000. An inherent biodegradability of FAT 40000 was determined in a 28 days Test according to the OECD Guideline for Testing of Chemicals, No. 302B. The test substance was tested in a concentration of 150 mg/l. The reference substance was diethylene glycol. Based on the results of the study, biodegradation of the test substance after 28 days was 0 %.

Description of key information

The test substance FAT 40000 was determined to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

A key study was performed to determine the percent biodegradation of FAT 40000. An inherent biodegradability of FAT 40000 was determined in a 28 days Test according to the OECD Guideline for Testing of Chemicals, No. 302B. The test substance was tested in a concentration of 150 mg/l. The reference substance was diethylene glycol. Based on the results of the study, biodegradation of the test substance after 28 days was 0 %.

 

The biochemical oxygen demand of FAT 40000/G is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20°C in different test concentrations diluted in water saturated with dissolved oxygen and containing a seed of microorganisms. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 mg/l. The BOD5 of FAT 40000/G: 3 mg 02/g The BOD5 of the reference substance is : 195,180, 200 mg O2/l.

 

A predeterminated amount of the test substance FAT 40000/G dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate (according to DEV H 41-1, DEV1980). The test substance concentration was tested in 5.0 mg/l. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. The COD of FAT 40000/G is : 402 mg 02/g The COD of the reference substance is: 193 mg O2/l.

 

Based on these results the corresponding BOD5/COD quotient is equivalent to zero, so no biodegradation is expected.

 

Other supporting studies were also performed with following results -

COD - 771 mg O2/g, 157 mg O2/g, 417 mg O2/g, 581 mg O2/g

BOD - 0 mg O2/g