Reaction mass of Trisodium 4-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-{[1-ethyl-2-hydroxy-4-methyl-6-oxo-5-(sulfonatomethyl)-1,6-dihydropyridin-3-yl]diazenyl}benzenesulfonate and Trisodium 4-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-{[1-ethyl-2-hydroxy-4-methyl-6-oxo-5-(sulfonatomethyl)-1,6-dihydropyridin-3-yl]diazenyl}benzenesulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study initiation date - 05 May 1993; Experiment start date - 11 May 1993; Experiment completion date - 13 May 1993; Study completion date - 05 August 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT 40000/G
Batch number: 67/92
Purity: 79.5%
Appearance: solid
Solubility: miscible
Storage: room temperature
Expiration date: 01 June 1993.

Analytical monitoring:

yes

Details on sampling:

All test medium samples for the analytical determinations were taken after mixing of the test solutions.

Vehicle:

yes

Remarks:

Bi-distilled water

Details on test solutions:

Test concentrations
Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/l nominal.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organism was the species Daphnia magna. The daphnids were bred in the laboratories of the Textile Dyes Division, Product Ecology TF under standardized conditions. For the test, animals with age of less than 24 hours were used. The quality of the daphnids will be checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate (range:0.8 -1.5 mg/l).
For each concentration 20 Daphnia magna were used.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

136 mg/l CaC03

Test temperature:

21 ± 1 °C

pH:

7.8 - 8.2.

Nominal and measured concentrations:

Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/l nominal.

Details on test conditions:

48 hours at 21 ± 1 °C, 16 h light/ 8 hours dark The daphnids were exposed to the test article in 250 ml beakers containing 100 ml test medium with 10 animals per beaker. All tests are running in duplicate. During exposure, the animals were kept at 21 ± 1 °C with 16 hours illumination.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 179.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 179.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

>= 179.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

In the control group, no immobilization of the daphnids was observed after 48 hours. In the lowest and in the highest test concentration of 60.6 mg/l and 179.1 mg/l, respectively, no toxic effect on the daphnids was found. Only in the test concentration of 102.0 mg/l one daphnia was immobilized. But this effect is probably not caused by the test substance.

Results with reference substance (positive control):

The EC50 value for the reference substance used was determined as 0.93 mg/l.

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 of FAT 40000 was determined to be >179.1 mg/L.

Executive summary:

The acute toxicity of FAT 40000/G to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemical, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations.

The nominal concentrations tested were 58, 100 and 180 mg test article/I, and a control without any additions. No immobility was observed during test duration in the control. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 99.1 - 104.7 % of the nominal values. Therefore, under the test conditions the test article was sufficiently stable.

In the control group, no immobilization of the daphnids was observed after 48 hours. In the lowest and in the highest test concentration of 60.6 mg/l and 179.1 mg/l, respectively, no toxic effect on the daphnids was found. Only in the test concentration of 102.0 mg/l one daphnia was immobilized. But this effect is probably not caused by the test substance.

Therefore, the EC50 values could not be calculated. The EC0 value is at least 179.1 mg/l, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 179.1 mg/l.

Description of key information

The 48 hour EC50 of FAT 40000/G was determined to be 179.1 mg/L..

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

179.1 mg/L

Additional information

The acute toxicity of FAT 40000/G to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemical, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations.

The nominal concentrations tested were 58, 100 and 180 mg test article/I, and a control without any additions. No immobility was observed during test duration in the control. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 99.1 - 104.7 % of the nominal values. Therefore, under the test conditions the test article was sufficiently stable.

In the control no immobilization of the daphnids was observed after 48 hours. In the lowest and in the highest test concentration of 60.6 mg/l and 179.1 mg/l, respectively, no toxic effect on the daphnids was found. Only in the test concentration of 102.0 mg/l one daphnia was immobilized. But this effect is probably not caused by the test substance.

Therefore, the EC50 values could not be calculated. The EC0 value is at least 179.1 mg/l, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 179.1 mg/l.