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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
1. Deion. water instead of dest. water for the stocksolution 2. Preparation of the inoculum :higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines) 3. 4 instead of 5 test concentrations
Principles of method if other than guideline:
None
GLP compliance:
yes
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
not specified
Details on test solutions:
None
Test organisms (species):
other: Activated sludge of a communal sewage treatment plant
Details on inoculum:
3 times washing with tap water and addition of synthetic sewage (a day before the main test)
Test type:
other:
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
20.6 °C
pH:
7.8 before the test beginning
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Details on test conditions:
320,100, 32 and 10 mg/l of the test substance
32,10 and 3.2 mg/l of the reference substance
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
None
Results with reference substance (positive control):
The IC50 for3,5- dichlorophenol was found to be 13.5 mg/L.
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
yes
Conclusions:
The IC50 for FAT 40000/G was found to be greater than 320 mg/L.
Executive summary:

The toxicity of FAT 40000/G to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline No. 209, and to the EG Method Nr.L 133/118. The test substance concentrations tested were 320,100, 32 and 10 mg/l. The reference substance 3,5-Dichlorophenol was tested in concentrations of 32,10 and 3.2 mg/l. The IC50 for reference substance was found to be 13.5 mg/L. Based on the findings, the IC50 for FAT 40000/G was found to be greater than 320 mg/L

Description of key information

The IC50 of the test substance FAT 40000 is greater than 320 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
320 mg/L

Additional information

The toxicity of FAT 40000 to activated sludge was determined in a 3-hour respiration inhibition test according to OECD Guideline 209. Based on the findings of the study, the IC50 of the test substance FAT 40000 is greater than 320 mg/L. Several other studies involving differnt batches of the test item have been conducted. In summary all came to the same results and conclusions which means that production prioces or batch related effects can be ruled out.

Several other supporting studies were also performed with following results:

IC50 = > 300 mg/l