Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: OECD Oxygen Consumption Test
Principles of method if other than guideline:
Method: German standard methods for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Nominal and measured concentrations:
10000 mg/l was highest concentration tested.
Details on test conditions:
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
Reference substance (positive control):
not specified
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: non-toxic at the limit of solubility
Details on results:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Validity criteria fulfilled:
yes
Conclusions:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
Method: DIN 38412, Teil 27 (Bacterial oxygen consumption test) method for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Nominal and measured concentrations:
10000 mg/l was highest concentration tested.
Details on test conditions:
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
Reference substance (positive control):
not specified
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: non-toxic at the limit of solubility
Details on results:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is 10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Validity criteria fulfilled:
yes
Conclusions:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is 10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Endpoint:
activated sludge respiration inhibition testing
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
OECD Oxygen Consumption Test
Principles of method if other than guideline:
Method: DIN 38412, Teil 27 (Bacterial oxygen consumption test) method for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.

GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Details on test conditions:
The oxygen consumption rate of a bacterial suspension fed glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identical conditions is also measured.
Reference substance (positive control):
not specified
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: non-toxic at the limit of solubility,
Details on results:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Description of key information

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Key value for chemical safety assessment

EC50 for microorganisms:
50 000 mg/L
EC10 or NOEC for microorganisms:
10 000 mg/L

Additional information

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L. The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.