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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Additional information on environmental fate and behaviour

Administrative data

Endpoint:
additional information on environmental fate and behaviour
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, non-guideline study following sound scientific principles. This is a non-standard, proprietary study specifically designed and conducted in order to provide supporting evidence to the testing program. Acceptable with restrictions.
Justification for type of information:
See IUCLID section 13 for category and read across justification

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Ecotoxicity screening studies were conducted in which a biomimetic extraction technique using solid-phase microextraction (SPME) in conjunction with gas chromatography (GC) was employed to assess the level of water soluble hydrocarbons and hence the potential toxicity of the water accommodated fractions (WAFs) of the test samples. In addition, the toxicity of WAFs was also monitored using an in-vitro MicrotoxTM assay, a biosensor-based measurement system.
GLP compliance:
no
Type of study / information:
Leaching of the thickener from base oils for a range of greases

Test material

Constituent 1
Reference substance name:
Lithium soaps
IUPAC Name:
Lithium soaps
Test material form:
other: Grease: thickener in a base oil

Results and discussion

Any other information on results incl. tables

Results and Conclusions

The SPME and Microtox results for the WAFs of the grease samples at 1000 mg/L loading rate are as follows:

Grease sample SPME-GC Microtox reading
(Total peak area) (5 min) (15 min)
Lithium soap grease 489,674 >100 >100
Lithium-calcium mixed soap grease 412,232 >100 >100
Lithium complex soap 1 548,207 >100 >100
Lithium complex soap 2 512,000 >100 >100

The metal analyses were as follows:

Grease sample Calcium (mg/L) Lithium (mg/L)
Lithium soap grease <0.1 <0.1
Lithium-calcium mixed soap grease <0.1 <0.1
Lithium complex soap 1 <0.1 <0.1
Lithium complex soap 2 <0.1 <0.1

Both sets of screening data confirm that there is no apparent toxicity associated with any of the samples at the 1000 mg/L loading rate. The SPME-GC data summarised above, supported by the chromatograms, confirm that the total peak area counts are all extremely low and equivalent to background readings associated with a control sample (i.e. a WAF without any test sample added).

All of the results for calcium and lithium analysis were below the limit of detection for the method, hence all results are reported as <0.1 mg/L.

The screening ecotoxicity data (using SPME-GC and the in-vitro Microtox assay), confirm a lack of toxicity of the greases.

Applicant's summary and conclusion

Conclusions:
There is negligible leaching of the thickeners from base oils in the samples tested. The SPME-GC data confirm that the total peak area counts are all extremely low and equivalent to background readings associated with a control sample. All of the results for calcium and lithium analysis were below the limit of detection for the method, hence all results are reported as <0.1 mg/L.
Executive summary:

Leaching studies were undertaken on thickeners in base oils to provide additional support to the grease thickener testing strategy. Information generated during the screening studies is of relevance and value to the risk assessment of a wide range of grease thickeners and to assess the potential bioaccessibility of grease and other lubricant components.

The approach for the organic components of the lithium soap thickeners was to use a solid-phase microextraction (SPME) method combined with gas chromatography (GC) to assess the bioaccessibility potential of the water accommodated fractions (WAFs) of lithium soap grease and this was complemented with metal ion analysis of the WAFs to determine whether the metal leaches out of the base grease during WAF preparation. Furthermore, ecotoxicity screening studies were conducted in order to determine whether there was any toxicity associated with the grease samples. The toxicity of WAFs was monitored using an in-vitro Microtox assay, a biosensor-based measurement system.

The screening ecotoxicity data (using SPME-GC and the in-vitro Microtox assay), confirm a lack of toxicity of the greases. Metal analyses confirm that there was no noticeable leaching of lithium or calcium ions from the grease samples.