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Administrative data

Endpoint:
additional toxicological information
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study is conducted according to an appropriate method, no mention of compliance with GLP.

Data source

Reference
Reference Type:
publication
Title:
Bioaccumulation and locomotor effects of manganese phosphate/sulfate mixture in Sprague-Dawley rats following subchronic (90 days) inhalation exposure
Author:
Salehi F, Krewski D, Mergler D, Normandin L, Kennedy G, Phillipe S & Zayed J
Year:
2003
Bibliographic source:
Toxicology and Applied Pharmacology 191 (2003) 264–271

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The objective of this study is to investigate exposure-response relationships for bioaccumulation and locomotor effects following subchronic inhalation exposure to a mixture of manganese phosphates/sulfate mixture. A control group and three groups of 30 male Sprague-Dawley rats were exposed in inhalation chambers for a period of 13 weeks, 5 days per week, 6 h a day. Exposure concentrations were 3000, 300, and 30 µg/m3. At the end of the exposure period, locomotor activity and resting time tests were conducted for 36 h using a computerized autotrack system. Rats were then euthanized by exsanguination and Mn concentrations in different tissues (liver, lung, testis, and kidney) and blood and brain (caudate putamen, globus pallidus, olfactory bulb, frontal cortex, and cerebellum) were determined by neutron activation analysis.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: fine crystalline powder
Details on test material:
Manganese phosphate/sulphate mixture, a fine crystalline powder, which includes Mn5(PO4)2(PO3(OH))24H2O hureaulite mineral form, and manganese (II) sulfate monohydrate (MnSO4 · H2O), were obtained from Alfa Aesar (Johnson Matthey Company) and combined 50/50 (wt/wt).

Results and discussion

Applicant's summary and conclusion

Conclusions:
Increased manganese concentrations were observed in blood, kidney, lung, testis, and in all brain sections in the highest exposure group. Mn in the
lung and in the olfactory bulb were dose dependent. Our data indicate that the olfactory bulb accumulated more Mn than other brain regions
following inhalation exposure. Locomotor activity was increased at 3000 µg/m3, but no difference was observed in resting time among the
exposed groups. At the end of the experiment, rats exposed to 300 and 3000 µg/m3 exhibited significantly decreased body weight in
comparison with the control group. Biochemical profiles also revealed some significant differences in certain parameters, specifically
alkaline phospatase, urea, and chlorate.