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Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to fish
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
α,2-dichlorotoluene
EC Number:
210-258-8
EC Name:
α,2-dichlorotoluene
Cas Number:
611-19-8
Molecular formula:
C7H6Cl2
IUPAC Name:
1-chloro-2-(chloromethyl)benzene
Details on test material:
IUCLID4 Test substance: A01-03:Wako Pure Chemical Industries, Ltd., Purity 99.6 %, Lot No. PAM5243

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Oryzias latipes

Study design

Test type:
flow-through

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.27 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

RS-Freetext:
[Measured Concentrations]
All of the measured concentrations were between 80 and 120%
of the nominal concentrations (Table 1). Thus the nominal
concentrations were used for calculating effect values.

Table 1.Measured concentrations of the test solutions in the
96-hour acute toxicity test on Oryzias latipes under the
flow-through test conditions
========================================================
Nominal     Measured concn.  Arithmetic  Measured/Nominal
concn.      (mg/l)           mean             (%)
            --------------------------------------------
(mg/l)      0 h     48 h    (mg/l)       0 h    48h
--------------------------------------------------------
Control     < 0.005 < 0.005  ---         ---    ---
Solv. cont. < 0.005 < 0.005  ---         ---    ---
0.10        0.100   0.101    0.101       100    101
0.18        0.158   0.183    0.171        88    102
0.32        0.368   0.287    0.328       115    90
0.56        0.543   0.513    0.528        97    92
1.0         0.971   1.05     1.01         97    105
========================================================


[Water Chemistry]
Table 2.pH values of the test solutions in the 96-hour acute
toxicity test on Oryzias latipes under the flow-through test
coditions
========================================================
      Cont.  Solv.           Nominal concn. (mg/l)
                      ----------------------------------
Hours        cont.    0.10   0.18   0.32  0.56   1.0
--------------------------------------------------------
0      7.2   7.2      7.2    7.2    7.2    7.2   7.2
24     7.4   7.3      7.3    7.3    7.3    7.3   7.2
48     7.3   7.3      7.3    7.2    7.2    7.2   7.2
72     7.2   7.2      7.2    7.1    7.1    7.1   ND
96     7.3   7.3      7.3    7.3    7.3    ND    ND
========================================================
ND: Not determined because all fishes were dead at this
time.


Table 3.Dissolved oxygen concentrations (DO) of the test
solutions in the 96-hour acute toxicity test on Oryzias
latipes under the flow-through test conditions
========================================================
      Cont.  Solv.          Nominal concn. (mg/l)
             cont.    ---------------------------------
Hours                 0.10   0.18  0.32  0.56   1.0
--------------------------------------------------------
0     8.8    8.8      8.6    8.7   8.8   8.8    8.7
24    8.5    8.6      8.5    8.5   8.7   8.6    8.7
48    8.5    8.6      8.5    8.6   8.6   8.6    8.8
72    9.0    8.9      8.9    8.9   8.9   9.1    ND
96    8.7    8.9      8.6    8.6   8.8    ND    ND
========================================================
ND: Not determined because all fishes were dead at this
time.

[Effect Data]
LC50 (96 hr)    : 0.27 mg/l(Table 4 & 5)
LC0 (96 hr)     : 0.18 mg/l(Table 4 & 6)
LC100 (96 hr)   : 0.56 mg/l(Table 4 & 6)
NOEC (96 hr)    : 0.18 mg/l(Table 4 & 7)


Table 4.Cumulative numbers of deaths in the 96-hour acute
toxicity test on Oryzias latipes under the flow-through test
conditions
========================================================
Nominal concn.   Cumulative number of deaths(a)
                 ----------------------------------------
(mg/l)           24hr     48hr      72hr      96hr
----------------------------------------------------------
Control          0 (0)    0 (0)     0 (0)     0 (0)
Solv. Control    0 (0)    0 (0)     0 (0)     0 (0)
0.10             0 (0)    0 (0)     0 (0)     0 (0)
0.18             0 (0)    0 (0)     0 (0)     0 (0)
0.32             0 (0)    0 (0)     4 (40)    8 (80)
0.56             1 (10)   8 (80)   10 (100)  10 (100)
1.0              2 (20)  10 (100)  10 (100)  10 (100)
=========================================================
(a):Percentage of dead animals compared to the total animals
tested is shown in parentheses.


Table 5.Calculated LC50 values based on the nominal
concentrations in the 96-hour acute toxicity test on Oryzias
latipes under the flow-through test conditions
========================================================
Exposure    LC50   95% confidence limit   Statistical 
Period      (mg/l)       (mg/l)           method
(hours)
--------------------------------------------------------
24        > 1.0       not calculated      Binominal
48          0.47      not calculated      Binominal
72          0.34      not calculated      Binominal
96          0.27      not calculated      Binominal
========================================================


Table 6.Maximum concentrations causing 0% mortality and
Minimum concentrations causing 100% mortality based on the
nominal concentrations in the acute toxicity test on
Oryzias latipes under the flow-through test conditions
===================================================
                                Exposure time
                       24 hr  48 hr  72 hr  96 hr
---------------------------------------------------
Maximum concn. (mg/l)  0.32   0.32   0.18   0.18
causing 0% mortality

Minimum concn. (mg/l)  > 1.0  1.0    0.56   0.56
causing 100% mortality
===================================================


Table 7.Visible abnormalities in the 96-hour acute toxicity
test on Oryzias latipes under the flow-through test
conditions
=======================================================
Nominal                            Symptom
concn.         ----------------------------------------
(mg/l)         24hr     48hr     72hr         96hr
-------------------------------------------------------
Control        Normal   Normal   Normal       Normal
Solv. Control  Normal   Normal   Normal       Normal
0.10           Normal   Normal   Normal       Normal
0.18           Normal   Normal   Normal       Normal
0.32           Normal   le       le, ss, es   es
0.56           le, ss   le, ss   ---a         ---a
1.0            le, ss   ---a     ---a         ---a
========================================================
le: Lethargy
ss: Surface slicks
es: Erratic swimming
a: No observation was made because all fishes were dead at
this time.

Applicant's summary and conclusion