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EC number: 210-258-8 | CAS number: 611-19-8
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
- GLP compliance:
- yes
Test material
- Reference substance name:
- α,2-dichlorotoluene
- EC Number:
- 210-258-8
- EC Name:
- α,2-dichlorotoluene
- Cas Number:
- 611-19-8
- Molecular formula:
- C7H6Cl2
- IUPAC Name:
- 1-chloro-2-(chloromethyl)benzene
- Details on test material:
- IUCLID4 Test substance: A01-03:Wako Pure Chemical Industries, Ltd., Purity 99.6 %, Lot No. PAM5243
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.02 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.23 mg/L
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 0.39 mg/L
Any other information on results incl. tables
RS-Freetext:
[Measured Concentrations]
Measured concentrations of the test solutions just after
renewal (fresh preparation) and those 48 hour after renewal
were ranged within 81 - 117% and 10 - 27% of the nominal
concentrations, respectively. Because all of the measured
concentrations at 48 hour after renewal were lower than 80%
of the nominal, the time-weighted concentrations were used
to calculate the effect values (Table 2 & 3).
Table 2.Measured concentrations of the test solutions in the 21-day reproduction test on Daphnia magna under the semi-static test conditions
========================================================
Nominal Measured Time Measured/Nominal
concn. Concn. -weighted (%)
(mg/l) (mg/l) mean
(mg/l)
---------------------------------------------
Day 0 Day 2 Day 0 Day 2
fresh old fresh old
--------------------------------------------------------
(Test 1)
Control <0.0001 <0.0001 --- --- ---
Solv. cont. <0.0001 <0.0001 --- --- ---
0.0022 0.00233 0.000212 0.000884 106 10
0.0046 0.00496 0.000559 0.00202 108 12
0.010 0.0107 0.00146 0.00464 107 15
0.022 0.0210 0.00324 0.00950 95 15
0.046 0.0528 0.00558 0.0210 115 12
0.10 0.0865 0.0143 0.0401 87 14
0.22 0.184 0.0326 0.0875 84 15
(Test 2)
Control <0.0001 <0.0001 --- --- ---
Solv. cont. <0.0001 <0.0001 --- --- ---
0.10 0.102 0.0187 0.0491 102 19
0.22 0.228 0.0466 0.114 104 21
0.46 0.484 0.113 0.255 105 25
1.0 1.16 0.226 0.571 116 23
========================================================
Table 2 - continued (1).
========================================================
Nominal Measured Time Measured/Nominal
concn. Concn. -weighted (%)
(mg/l) (mg/l) mean
(mg/l)
----------------------------------------------
Day 6 Day 8 Day 6 Day 8
fresh old fresh old
---------------------------------------------------------
(Test 1)
Control <0.0001 <0.0001 --- --- ---
Solv. cont. <0.0001 <0.0001 --- --- ---
0.0022 0.00241 <0.0001 0.000499 110 ---
0.0046 0.00411 0.000663 0.00189 89 14
0.010 0.0109 0.000966 0.00410 109 10
0.022 0.0216 0.00311 0.00954 98 14
0.046 0.0373 0.00678 0.0179 81 15
0.10 0.0952 0.0144 0.0428 95 14
0.22 0.222 0.0334 0.0996 101 15
(Test 2)
Control <0.0001 <0.0001 --- --- ---
Solv. cont. <0.0001 <0.0001 --- --- ---
0.10 0.0959 0.0220 0.0502 96 22
0.22 0.219 0.0526 0.117 100 24
0.46 0.540 0.126 0.284 117 27
=========================================================
Table 2 - continued (2).
=========================================================
Nominal Measured Time Measured/Nominal
concn. Concn. -weighted (%)
(mg/l) (mg/l) mean
(mg/l)
-----------------------------------------------
Day 14 Day 16 Day 14 Day 16
fresh old fresh old
---------------------------------------------------------
(Test 1)
Control <0.0001 <0.0001 --- --- ---
Solv. cont. <0.0001 <0.0001 --- --- ---
0.0022 0.00213 0.000245 0.000872 97 11
0.0046 0.00448 0.000603 0.00193 97 13
0.010 0.00873 0.00166 0.00426 87 17
0.022 0.0237 0.00381 0.0109 108 17
0.046 0.0420 0.00754 0.0201 91 16
0.10 0.0916 0.0140 0.0413 92 14
0.22 0.240 0.0388 0.110 109 18
(Test 2)
Control <0.0001 <0.0001 --- --- ---
Solv. cont. <0.0001 <0.0001 --- --- ---
0.10 0.0988 0.0210 0.0502 99 21
0.22 0.218 0.0533 0.117 99 24
0.46 0.512 0.126 0.275 111 27
=======================================================
Table 3.The time-weighted mean values for the measured
concentrations of test solutions in periods during the 21-day reproduction test on Daphnia magna under the semi-static test conditions
========================================================
Nominal Time-weighted mean value Mean Value/Nominal
concn. (mg/l) (%)
(mg/l)
--------------------------------------------------
0-7d. 0-14d. 0-21d. 0-7d. 0-14d. 0-21d.
----------------------------------------------------------
(Test 1)
0.0022 0.000884 0.000691 0.000751 40 31 34
0.0046 0.00202 0.00195 0.00195 44 42 42
0.010 0.00464 0.00437 0.00433 46 44 43
0.022 0.00950 0.00952 0.00997 43 43 45
0.046 0.0210 0.0195 0.0197 46 42 43
0.10 0.0401 0.0414 0.0414 40 41 41
0.22 0.0875 0.0935 0.0992 40 43 45
(Test 2)
0.10 0.0491 0.0496 0.0498 49 50 50
0.22 0.114 0.115 0.116 52 52 53
0.46 0.255 0.270 0.272 55 59 59
1.0 0.571 ---a ---a 57 ---a ---a
========================================================
d.: days
a:All of the parental animals died before Day 21 and thus
the time-weighted mean value was not calculated for the
period.
[Water Chemistry]
pH: 7.8 - 8.7 (Test 1), 7.8 - 8.8 (Test 2)
Dissolved oxygen (mg/l): 8.4 - 9.8 (Test 1),
8.6- 9.7 (Test 2)
Total hardness (mg/l as CaCO3): 87 - 88 (Test 1),
85 - 88 (Test 2)
RS-Freetext:
[Effect Data (reproduction)]
All of the following effect values were calculated with the
measured concentrations. NOEC and LOEC values were
determined on the basis of reproduction using the data of
Test 1.
LC50 (21 days) = 0.39 mg/l (parental mortality)
(Table 4 & 5)
EC50 (21 days) = 0.23 mg/l
(95% Confidence limits: 0.22 - 0.24 mg/l)
(Table 7 - 9)
NOEC (21 days) = 0.020 mg/l (Table 7 & 8)
LOEC (21 days) = 0.041 mg/l (Table 7 & 8)
Table 4.Cumulative numbers of deaths among the parental
animal groups
=======================================================
Nominal Cumulative number of deaths on Day:
concn. ----------------------------------------
(mg/l) 0 1 2 3 4 5 6 7 8 9 10
-------------------------------------------------------
(Test 1)
Control 0 0 0 0 0 0 0 0 0 0 0
Solv. cont. 0 0 0 0 0 0 0 0 0 0 0
0.0022 0 0 0 0 0 0 0 0 0 0 0
0.0046 0 0 0 0 0 0 0 0 0 0 0
0.010 0 0 0 0 0 0 0 0 0 0 0
0.022 0 0 0 0 0 0 0 0 0 0 0
0.046 0 0 0 0 0 0 0 0 0 0 0
0.10 0 0 0 0 0 0 0 0 0 0 0
0.22 0 0 0 0 0 0 0 0 0 0 0
(Test 2)
Control 0 0 0 0 0 0 0 0 0 0 0
Solv. cont. 0 0 0 0 0 0 0 0 0 0 0
0.10 0 0 0 0 0 0 0 0 0 0 0
0.22 0 0 0 0 0 0 0 0 0 0 0
0.46 0 0 0 0 0 0 0 0 0 0 0
1.0 0 0 0 3 10 10 10 10 10 10 10
===================================================
Table 4 - continued.
======================================================
Nominal Cumulative number of deaths on Day:
concn. ------------------------------------------
(mg/l) 11 12 13 14 15 16 17 18 19 20 21
---------------------------------------------------------
(Test 1)
Control 0 0 0 0 0 0 0 0 0 0 0
Solv. cont. 0 0 0 0 0 0 0 0 0 0 0
0.0022 0 0 0 0 0 0 0 0 0 0 0
0.0046 0 0 0 0 0 0 0 0 0 0 0
0.010 0 0 0 0 0 0 0 0 0 0 0
0.022 0 0 0 0 0 0 0 0 0 0 0
0.046 0 0 0 0 0 0 0 0 0 0 0
0.10 0 0 0 0 0 0 0 0 0 0 0
0.22 0 0 0 0 0 0 0 0 0 0 0
(Test 2)
Control 0 0 0 0 0 0 0 0 0 0 0
Solv. cont. 0 0 0 0 0 0 0 0 0 0 0
0.10 0 0 0 0 0 0 0 0 0 0 0
0.22 0 0 0 0 0 0 0 0 0 0 0
0.46 0 0 0 0 0 0 0 0 0 0 0
1.0 10 10 10 10 10 10 10 10 10 10 10
=========================================================
Table 5.Cumulative mortality of parental animal groups (%)
=========================================================
Nominal Cumulative percentage of deaths on
concn. Day:
------------------------------------------
(mg/l) 1 2 4 7 14 21
---------------------------------------------------------
(Test 1) 0 0 0 0 0 0
Control 0 0 0 0 0 0
Solv. cont. 0 0 0 0 0 0
0.0022 0 0 0 0 0 0
0.0046 0 0 0 0 0 0
0.010 0 0 0 0 0 0
0.022 0 0 0 0 0 0
0.046 0 0 0 0 0 0
0.10 0 0 0 0 0 0
0.22 0 0 0 0 0 0
(Test 2)
Control 0 0 0 0 0 0
Solv. cont. 0 0 0 0 0 0
0.10 0 0 0 0 0 0
0.22 0 0 0 0 0 0
0.46 0 0 0 0 0 0
1.0 0 0 100 100 100 100
=========================================================
Table 6.The time of the first production of juveniles (days)
=======================================================
Nominal Bottle number
concn. ---------------------------------------
(mg/l) 1 2 3 4 5 6 7 8 9 10 Mean
-------------------------------------------------------
(Test 1)
Control 8 8 8 9 8 8 8 8 8 8 8.1
Solv. cont. 8 8 8 8 8 8 8 8 9 9 8.2
0.0022 8 8 8 8 8 8 9 8 8 9 8.2
0.0046 9 8 8 8 8 9 8 8 8 9 8.3
0.010 8 8 9 8 9 9 8 8 8 8 8.3
0.022 8 8 8 9 8 8 8 9 9 8 8.3
0.046 8 8 8 8 9 8 9 8 8 8 8.2
0.10 8 8 8 8 8 8 8 8 9 8 8.1
0.22 8 8 9 8 8 8 8 8 8 8 8.1
(Test 2)
Control 8 8 8 8 8 8 8 8 8 9 8.1
Solv. cont. 8 8 8 8 9 8 8 8 9 8 8.2
0.10 8 8 8 8 8 8 8 8 8 8 8.0
0.22 10 10 8 8 9 8 9 8 8 10 8.1
0.46 15 11 13 12 11 10 13 11 12 13 12.1
1.0 D D D D D D D D D D ---a
=====================================================
D: The parental animal died before producing juveniles.
a:No production of juveniles during the 21-day test period.
Table 7.Mean cumulative numbers of juveniles produced per
parental
animal survived for 21 days
=======================================================
Nominal Mean cumulative number on
concn. Day:
--------------------------------------------------
(mg/l) 0 7 8 9 10 11 12 13
----------------------------------------------------------
(Test 1)
Control 0.0 0.0 4.2 4.9 4.9 25.5 29.0 29.0
Solv. cont.
0.0 0.0 3.2 3.8 3.8 21.0 25.2 25.2
0.0022 0.0 0.0 5.1 6.7 6.7 23.6 28.2 28.2
0.0046 0.0 0.0 4.8 6.4 6.4 22.2 28.2 28.2
0.010 0.0 0.0 4.6 6.2 6.2 24.4 31.1 31.1
0.022 0.0 0.0 3.8 5.6 5.6 20.9 28.0 28.0
0.046 0.0 0.0 5.1 5.9 5.9 22.6 27.5 27.5
0.10 0.0 0.0 0.2 0.2 0.2 12.2 13.4 13.4
0.22 0.0 0.0 0.0 0.0 0.0 8.3 9.0 9.0
(Test 2)
Control 0.0 0.0 9.0 10.5 10.5 29.0 35.4 35.4
Solv. cont.
0.0 0.0 5.9 8.1 8.1 24.7 31.5 31.5
0.10 0.0 0.0 10.4 10.4 10.4 31.5 31.5 31.5
0.22 0.0 0.0 3.3 5.4 9.0 19.0 24.0 33.8
0.46 0.0 0.0 0.0 0.0 0.8 3.3 5.0 7.1
1.0 0.0 ---a ---a ---a ---a ---a ---a ---a
=======================================================
Table 7 - continued.
=======================================================
Nominal Mean cumulative number on
concn. Day:
-----------------------------------------------
(mg/l) 14 15 16 17 18 19 20 21
-------------------------------------------------------
(Test 1)
Control 53.3 56.8 56.8 84.6 88.0 88.0 96.6 115.5
Solv. cont.
49.9 55.7 55.7 81.2 87.4 87.4 95.6 115.0
0.0022 53.9 60.4 60.4 82.4 87.0 87.0 103.4 110.0
0.0046 50.7 61.0 61.0 78.9 88.7 88.7 105.7 116.9
0.010 56.3 67.6 67.6 88.3 97.5 97.5 112.1 121.7
0.022 51.0 59.7 59.7 79.9 87.5 87.5 102.9 112.8
0.046 52.0 58.2 58.2 80.6 86.0 86.0 106.9 112.4
0.10 39.3 41.1 41.1 64.5 67.1 67.1 91.6 94.6
0.22 32.8 35.2 35.2 56.9 59.4 59.4 84.1 87.0
(Test 2)
Control 57.8 63.3 63.3 86.0 91.6 91.6 108.4 115.2
Solv. cont.
53.6 63.5 63.5 86.3 93.7 93.7 102.0 122.6
0.10 62.6 62.6 62.6 87.3 87.3 87.3 111.5 114.0
0.22 47.2 51.4 57.6 72.7 78.6 88.7 101.1 101.1
0.46 12.3 16.3 21.2 28.9 34.8 37.0 40.7 45.3
1.0 ---a ---a ---a ---a ---a ---a ---a ---a
======================================================
a:Not counted because the parental animal died before
producing juveniles.
Table 8.Cumulative numbers of juveniles per parental animal
survived for 21 days in Test 1.
=======================================================
Nominal concn., mg/l
Bottle (Measured concn., mg/l)
No. --------------------------------------------------
Control Solv. cont. 0.0022 0.0046 0.010
(0.000751) (0.00195) (0.00433)
---------------------------------------------------------
1 126 105 103 124 109
2 113 112 111 110 122
3 115 112 112 111 102
4 113 116 110 119 100
5 113 131 108 115 114
6 122 114 115 126 146
7 108 124 109 116 143
8 116 106 118 107 122
9 121 116 106 123 109
10 108 114 108 118 131
--------------------------------------------------------
Mean 115.5 115.0 110.0 116.9 121.7
S.D. 5.9 7.7 4.3 6.3 14.6
--------------------------------------------------------
Inhibition
rate(%)
--- 0.4 4.8 -1.2 -5.4
--------------------------------------------------------
Significant difference
--- NS NS NS NS
=======================================================
Table 8 - continued.
=======================================================
Nominal concn., mg/l
Bottle (Measured concn.,mg/l)
No. ------------------------------------------
0.022 0.046 0.10 0.22
(0.00997) (0.0197) (0.0414) (0.0992)
-------------------------------------------------------
1 103 118 84 94
2 119 105 84 82
3 99 115 106 85
4 100 109 103 86
5 118 113 96 85
6 124 107 93 96
7 114 115 107 86
8 110 110 87 86
9 111 115 86 85
10 130 117 100 85
-------------------------------------------------------
Mean 112.8 112.4 94.6 87.0
S.D. 10.3 4.4 9.1 4.4
-------------------------------------------------------
Inhibition 2.3 2.7 18.1 24.7
rate (%)
-------------------------------------------------------
Significant NS NS ## ##
difference(a)
=======================================================
(a)Indicates a significant difference from the control
determined by the Dunnett's multicomparisons procedure,
one-sided test.
NS : not significant (p >= 0.05)
# : significant (p < 0.05)
## : significant (p < 0.01)
Table 9.Cumulative numbers of juveniles per parental animal
survived for 21 days in Test 2.
========================================================
Nominal concn., mg/l
Bottle (Measured concn.,mg/l)
No. ---------------------------------------------
Cont. S. C. 0.10 0.22 0.46 1.0
(0.0498) (0.116)(0.272) (0.571)
----------------------------------------------------------
1 104 116 114 107 34 D
2 109 123 115 106 43 D
3 129 123 104 108 45 D
4 110 118 114 121 53 D
5 117 115 111 77 50 D
6 108 110 123 94 41 D
7 117 129 132 95 39 D
8 120 128 117 119 74 D
9 111 123 100 100 42 D
10 127 141 110 84 32 D
---------------------------------------------------------
Mean 115.5 122.6 114.0 101.1 45.3 ---
S.D. 8.3 8.8 9.0 14.1 11.9 ---
---------------------------------------------------------
Inhibition --- -6.4 1.0 12.2 60.7 ---
rate (%)
---------------------------------------------------------
Significant --- NS NS ## ## ---
difference(a)
=========================================================
D: Not calculated because the parental animal was dead
before producing juveniles.
(a)Indicates the significant difference from the control by
Dunnett's multicomparison procedure, one-sided test.
NS : not significant (p >= 0.05)
# : significant (p < 0.05)
## : significant (p < 0.01)
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