α,2-dichlorotoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

α,2-dichlorotoluene

EC Number:

210-258-8

EC Name:

α,2-dichlorotoluene

Cas Number:

611-19-8

Molecular formula:

C7H6Cl2

IUPAC Name:

1-chloro-2-(chloromethyl)benzene

Details on test material:

Source: Wako Pure Chemical Industries, Ltd.,
Purity 99.6 %,
Lot No. PAM5243

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data available

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

no data available

Test solutions

Vehicle:

yes

Details on test solutions:

Solvent/Dispersant and Concentrations: Mixture of dimethylsulfoxide and polyoxyethylenesorbitan fatty acid ester, 100 µl/l test solution

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

a) Age: < 24 hours old
b) Supplier/Source: National Institute for Environmental Studies (JAPAN)

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no data available

Test conditions

Hardness:

75 mg/L (as CacO3)

Test temperature:

20.0 - 20.1 °C

pH:

7.6 - 7.8

Dissolved oxygen:

8.6 - 9.0

Salinity:

no data available

Nominal and measured concentrations:

Nominal Concentrations (as mg/l): 0, 0.10, 0.18, 0.32, 0.56 and 1.0
All measured concentrations were between 80 and 120% of the nominal concentrations. Thus the nominal concentrations were used for calculating effect values.

Details on test conditions:

Exposure Vessel Type: 9-liter test solution in a 10-liter glass vessel
Number of Replicates: 4
Individuals per Replicates: 5
Renewal Rate of Test Water: Flow-through with a flow-rate of 50 ml/min, equivallent to 8-times renewal per day
Light Condition: 16:8 hours, light-darkness cycle
Feeding: No

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.38 mg/L

Any other information on results incl. tables

Table 1.Measured concentrations of the test solutions in the
48-hour acute immobilisation test on Daphnia magna under the
flow-through test conditions
========================================================
Nominal       Measured          Arithmetic  Measured
concn.        concn.            mean        /Nominal
(mg/l)       (mg/l)             (mg/l)       (%)
              --------------                ------------
              0 h      48 h                 0 h   48 h
--------------------------------------------------------
Control       <0.005  <0.005     ---        ---   ---
Solv. Control <0.005  <0.005     ---        ---   ---
0.10          0.111    0.112     0.112      111   112
0.18          0.214    0.198     0.206      119   110
0.32          0.359    0.284     0.322      112    89
0.56          0.622    0.544     0.583      111    97
1.0           1.10     1.01      1.06       110   101
=========================================================


[Water Chemistry]
Table 2.pH values of the test solutions in the 48-hour acute
Immobilisation test on Daphnia magna under the flow-through
test conditions
==========================================
Nominal concn.               pH
                    ----------------------
(mg/l)              0 hour     48 hours
------------------------------------------
Control             7.8        7.7
Solvent control     7.8        7.7
0.10                7.8        7.7
0.18                7.8        7.7
0.32                7.8        7.7
0.56                7.8        7.7
1.0                 7.8        7.6
==========================================


Table 3.Dissolved oxygen concentrations (DO) of the test
solutions in the 48-hour acute immobilisation test on
Daphnia magna under the flow-through test conditions
===========================================
Nominal concn.             DO (mg/l)
                     ----------------------
(mg/l)               0 hour     48 hours
-------------------------------------------
Control              8.9        9.0
Solvent control      8.8        9.0
0.10                 8.7        8.9
0.18                 8.7        8.9
0.32                 8.6        8.9
0.56                 8.6        8.9
1.0                  8.6        8.9
===========================================

[Effect Data]
EiC50 (24 hr) : 0.72 mg/l   (Table 4 & 5)
EiC50 (48 hr) : 0.38 mg/l
    (95% confidence limits: 0.33 - 0.45mg/l)(Table 4 & 5)
EiC100 (48 hr) : 1.0 mg/l   (Table 4 & 6)
NOECi (48 hr)  : 0.10 mg/l  (Table 4 & 6)

Table 4.Cumulative numbers of deaths or immobility on Daphnia magna
=========================================================
Nominal concn.     Cumulative number of deaths or immobility
                  (Cumulative % of deaths or immobility)
                   --------------------------------------
(mg/l)             24 hours   48hours
---------------------------------------------------------
Control            0 (0)      0 (0)
Solvent. Control   0 (0)      0 (0)
0.10               0 (0)      0 (0)
0.18               0 (0)      1 (5)
0.32               0 (0)      4 (20)
0.56               1 (5)     18 (90)
1.0                2 (100)   20 (100)
=========================================================

Table 5.Calculated EiC50 values based on the nominal concentrations
========================================================
Exposure    EiC50   95% confidence limits   Statistical
Period                                      method
(hours)     (mg/l)      (mg/l)
--------------------------------------------------------
24          0.72      not calculated        Binominal
48          0.38      0.33 - 0.45           Probit
=========================================================

Table 6.No observed effective concentration (NOEC) and the
lowest concentration in 100% mortality or immobility based
on the nominal concentrations
====================================================
Exposure   NOEC     Lowest concentration in 100%
Period              mortality or immobility
(hours)   (mg/l)    (mg/l)
----------------------------------------------------
24         0.32     1.0
48         0.10     1.0
====================================================

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 of Daphnia magna was determiend to be 0.38 mg/L OCBC.

Executive summary:

OCBC was assessed in an acute toxicity test to Daphnia magna, according to OECD guideline 202. Daphnids were exposed in a flow-through test to the test item for 48 hours at nominal concentrations of 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L. The 48 h EC50 was determined to be 0.38 mg/L.