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EC number: 210-258-8 | CAS number: 611-19-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11.03.1987 - 03.07.1987
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- α,2-dichlorotoluene
- EC Number:
- 210-258-8
- EC Name:
- α,2-dichlorotoluene
- Cas Number:
- 611-19-8
- Molecular formula:
- C7H6Cl2
- IUPAC Name:
- 1-chloro-2-(chloromethyl)benzene
- Details on test material:
- Source: HOECHST AG- Code: GLAC 405- Purity: >99%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Wistar, Hoe: WISKf(SPF71)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: HOECHST AG, SPF-breeding
- Age at study initiation: 8 - 10 weeks
- Weight at study initiation:
males: mean 201 g (217 - 184 g), n =30
females: mean 199 g (209 - 177 g), n =30
- Fasting period before study:
- Housing: 5 rats of same sex in Makrolon cages with softwood granulate material
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: not less than 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2°C
- Humidity (%): 50 +/- 20%
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Stainless steel-glass cylinder
- Exposure chamber volume: 60 L
- Method of holding animals in test chamber: zylindric cages, headnose exposition
- Source and rate of air: 800 L/h
- Method of conditioning air: substance injection into the spray valve:
Group 1: 2 mL/h
Group 2: 6 mL/h
Group 3: 12 mL/h
Group 4: 22.5 mL/h
Group 5: 60 mL/h
Group 6/7: 90 mL/h
- System of generating particulates/aerosols: Compressed air (pressure: 4 bar) passed a spray valve (800 L/h) to obtain a primary aerosol
in a 10 L four-necked round-bottomed flask. Secondary aerosol reached the inhalation chamber by a rise pipe.
Air is exhausted at the bottom of the inhalation chamber.
- Method of particle size determination: no data
- Treatment of exhaust air: different filter and washing bottles (10% NaOH, CaCl2)
- Temperature, humidity, pressure in air chamber: modest low pressure (aeration: 800 L/h; exhaust: 1100 L/h)
TEST ATMOSPHERE
- Brief description of analytical method used:
The active ingredient is absorbed in n-hexane. N-hexane is cooled by a mixture of methanol/carbondioxide.
The hexane solution is analyzed with GC-FID, using an external standardization.
- Samples taken from breathing zone: no data - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0.587, 1.548, 1.648, 2.716, 5.268 or 5.723 (4.545) mg/L o-Chlorobenzylchloride
The value 4.545 mg/L was replaced by the value 5.732 mg/L, which was used for LC50 calculation.
It was obtained under identical conditions, but without animals (group 7). - No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- 30 male and 30 female Wistar rats were used. Rats were allocated to 1 of 6 dose groups, each of 5 males and 5 females.
The rats were observed during exposure and at least twice daily throughout the 14-day observation period.
During the observation period all rats were weighed at day 7 and 14 post treatment.
At the end of the 14-day observation period, the rats were sacrificed by an overdose of Nembutal except
those animals which showed ongoing symptoms.
Dead and sacrificed rats were subjected to a detailed macroscopic examination. - Statistics:
- Probit analysis
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 2.8 mg/L air
- Based on:
- test mat.
- 95% CL:
- 1.98 - 4.18
- Exp. duration:
- 4 h
- Mortality:
- Dose 0.587 mg/L: males 0/5; females 0/5 : cumulative 0/10
Dose 1.548 mg/L: males 1/5; females 2/5 ; cumulative 3/10
Dose 1.648 mg/L: males 3/5; females 2/5 ; cumulative 5/10
Dose 2.716 mg/L: males 1/5; females 1/5 ; cumulative 2/10
Dose 5.268 mg/L: males 3/5; females 3/5 ; cumulative 6/10
Dose 5.723 mg/L: males 5/5; females 5/5 ; cumulative 10/10
Deaths occurred in between day 1 and day 44 post beginning of treatment. - Clinical signs:
- other: Gasping respiration, respiratory sounds, uncoordinated, ataxic and stilted gait, cyanosis, stupor, squatting posture, prone position, flanks pinched in, nose and lid margin red-encrusted, corneal opacity, and narrow palpebra fissure. Except two females of
- Body weight:
- All surviving animals had exceeded their primary weights.
- Gross pathology:
- Macroscopic examination of perished rats revealed red coloured lungs. Pulmonary section resulted in discharge of a clear fluid and of foam.
Sporadically beige spots on liver and inflated small intestine were observed.
Except the two females killed at day 56 none of the rats sacrificed at the end of the observation period showed any macroscopic abnormalities.
Any other information on results incl. tables
Table 1. Mortality during observation period:
=======================================================
Dose (mg/l) males females cumulative
0.587 0 / 5 0 / 5 0 / 10
1.548 1 / 5 2 / 5 3 / 10
1.648 3 / 5 2 / 5 5 / 10
2.716 1 / 5 1 / 5 2 / 10
5.268 3 / 5 3 / 5 6 / 10
5.723 5 / 5 5 / 5 10 / 10
=======================================================
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information R20: Harmful by inhalation / GHS: Cat. 4 Criteria used for interpretation of results: EU
- Conclusions:
- LC50 Combined = 2.80 mg o-Chlorobenzylchloride/L air
- Executive summary:
A study on acute inhalation toxicity in rats was carried out under OECD GL 403 in compliance with GLP. Rats (5 animals/sex/group) were exposed (headnose) continuously for 4 hours to o-Chlorobenzylchloride aerosol at concentrations of 0.587, 1.548, 1.648, 2.716, 5.268 and 5.723 mg/L, and observed for 14 days. Death occurred at 1.548 mg/L and higher. The LC50 value was estimated to be 2.8 mg/L in both sexes. Clinical signs observed were gasping respiration, respiratory sounds, uncoordinated, ataxic and stilted gait, cyanosis, stupor, squatting posture, prone position, flanks pinched in, nose and lid margin red-encrusted, corneal opacity, and narrow palpebra fissure.
LC50 Males = 2.80 mg/L (confidence level p=0.05: 1.55 – 5.48 mg o-Chlorobenzylchloride/L air)
LC50 Females = 2.80 mg/L (confidence level p=0.05: 1.55 – 5.47 mg o-Chlorobenzylchloride/L air)
LC50 Combined = 2.80 mg/L (confidence level p=0.05: 1.98 – 4.18 mg o-Chlorobenzylchloride/L air)
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