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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-and OECD testing guideline compliant study with well characterised test material

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Qualifier:
according to guideline
Guideline:
OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
Principles of method if other than guideline:
Method: other: OECD Test Guidelines 471 and 472 "Genetic Toxicology (Salmonella typhimurium and Escherichia coli)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
4-aminotoluene-3-sulphonic acid
EC Number:
201-831-3
EC Name:
4-aminotoluene-3-sulphonic acid
Cas Number:
88-44-8
Molecular formula:
C7H9NO3S
IUPAC Name:
2-amino-5-methylbenzene-1-sulfonic acid
Details on test material:
Mitsuboshi Chemical Co., Ltd.: purity >99%

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium (TA100, TA1535, TA98, TA1537); Escherichia coli (WP2uvrA)
Metabolic activation:
with and without
Metabolic activation system:
S9 from male rat liver, induced with phenobarbital and 5,6-benzoflavone
Test concentrations with justification for top dose:
-S9mix and +S9mix: 0, 313, 625, 1250, 2500, 5000 ug/plate
Vehicle / solvent:
S9 from male rat liver, induced with phenobarbital and 5,6-benzoflavone
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: See details in test conditions below
Details on test system and experimental conditions:
number of replicate: 2
plates per test: 3
application: pre-incubation

positive control groups and the solvent:
without S9 mix; 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (TA98, TA100, WP2; DMSO), sodium azide (TA1535; pure water), 9-aminoacridine
(TA1537; DMSO)
with S9 mix; 2-aminoanthracene (all five strains; DMSO)

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium (TA100, TA1535, TA98, TA1537); Escherichia coli (WP2uvrA)
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Salmonella typhimurium            Escherichia coli
     TA100, TA1535, TA98, TA1537           WP2 uvrA
              +      ?      -             +      ?      -
-S9 mix:    [   ]  [   ]  [ * ]         [   ]  [   ]  [ * ]
+S9 mix:    [   ]  [   ]  [ * ]         [   ]  [   ]  [ * ]

In each experiment, the positive control chemicals induced
the expected responses, indicating that the assay was
working satisfactorily.

Applicant's summary and conclusion

Conclusions:
This substance was not mutagenic to Salmonella typhimurium
and Escherichia coli.