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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: OECD guideline used

Data source

Reference
Title:
Unnamed
Year:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
yes
Remarks:
Not limit test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-aminotoluene-3-sulphonic acid
EC Number:
201-831-3
EC Name:
4-aminotoluene-3-sulphonic acid
Cas Number:
88-44-8
Molecular formula:
C7H9NO3S
IUPAC Name:
2-amino-5-methylbenzene-1-sulfonic acid
Details on test material:
Wako Pure Chemical Industries, Ltd.: purity >95%

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Limit test:
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC0
Effect conc.:
> 10 mg/L
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 10 mg/L
Basis for effect:
growth rate

Any other information on results incl. tables

CONCENTRATIONS
nominal            measured
concent-       concentration (mg/L)         geometric mean
ration (mg/L)  0hr (%)  72hr (%)     0-72hr  24-48hr 24-72hr
------------------------------------------------------------
control          <0.1      <0.1         <0.1    <0.1    <0.1
solvent control  <0.1      <0.1         <0.1    <0.1    <0.1
  10            9.6 (96)   7.9 (79)      8.5      8.5      8.5

EFFECT DATA (based on limit test)
biomass method
 EbC50 (0-72hr)   > 10mg/L
 NOECb (0-72hr)  = 10mg/L
growth rate method
 ErC50 (24-48hr)  > 10mg/L
 NOECr (24-48hr) = 10mg/L
 ErC50 (24-72hr)  > 10mg/L
 NOECr (24-72hr) = 10mg/L

AVERAGE CELL DENSITY DURING 72HR EXPOSURE
nominal concent-      cell density (x10E4 cells/mL)
ration (mg/L)        0hr      24hr      48hr      72hr
------------------------------------------------------------
control             1.00      4.68      26.3      159
solvent control     1.00      4.73      26.3      161
  10                1.00      4.79      26.3      173

AVARAGE GROWTH INHIBITION
nominal concent-     biomass      growth rate    growth rate
ration (mg/L)        (0-72hr) %     (24-48hr) %    (24-72hr)

------------------------------------------------------------
control                0                0               0
solvent control       -0.7              0.7             0.1
  10                  -6.4              1.5            -1.6

MONITORING DATA
water temperature: 23.4-23.8°C
pH: 7.3-7.7 at start, 7.6 at end
intensity of irradiation: 4000-5000 lux

REMARK
This study was limit test at 10 mg/L only, due to author's
misunderstanding to the solubility in water. Therefore it was not a real limit test.

Applicant's summary and conclusion

Conclusions:
No growth inhibition was observed to green algae up to
10mg/L concentration.