4-aminotoluene-3-sulphonic acid

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: OECD guideline used

Data source

Reference

Title:

Unnamed

Year:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-aminotoluene-3-sulphonic acid

EC Number:

201-831-3

EC Name:

4-aminotoluene-3-sulphonic acid

Cas Number:

88-44-8

Molecular formula:

C7H9NO3S

IUPAC Name:

2-amino-5-methylbenzene-1-sulfonic acid

Details on test material:

Wako Pure Chemical Industries, Ltd.: purity >95%

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

3.2 mg/L

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

10 mg/L

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Effect concentrations 4

Duration:

21 d

Dose descriptor:

other: EC0

Effect conc.:

> 10 mg/L

Any other information on results incl. tables

CONCENTRATIONS
nominal        measured concentration (mg/L) (% of nominal) 
concent- 
ration         2day  5day  9day  12day  16day  19day  21day
(mg/L)         new   old   new    old     new     old   mean
-----------------------------------------------------------
control        <0.1   <0.1  <0.1  <0.1   <0.1   <0.1
solvent
control        <0.1   <0.1  <0.1  <0.1   <0.1   <0.1
1.0              1.0     1.0    0.90   1.1    1.0    1.1     1.0
                (100)  (100)  (90)  (110) (100)  (110)   (100)
3.2              3.1     3.3    2.9    3.5    3.2    3.5     3.2
                (97)  (100)  (91)   (110)  (100)  (110)   (100)
10               9.7     10     9.1    11     10     11     10
                (97)  (100)  (91)   (110) (100)  (110)    (100)
------------------------------------------------------------
rem. new = fresh solution
     old = expired solution
    mean = time-weighted mean during 21 days
As the result measured concentration was equivalent to
nominal ones.
OBSERVATION
mortality: No dead parental daphnia was observed in any dose
levels.
first brood day: First brood day of 1.0 (7-10 day), 3.2
(7-10 day) and 10 (7-8 day) mg/L were equivalent to
control(7 day) and solvent control (7-10 day).

MEAN CUMULATIVE NUMBER OF JUVENILES PRODUCED PER ADULT
nominal concent-     No. of juveniles
ration (mg/L)        at day 21 (mean)
------------------------------------------------------
control                  112.9
solvent control      109.1
1.0                      108.6
3.2                      99.1
10                       74.3*
------------------------------------------------------
rem. * significant different (p=0.01) from solvent control

No other change was observed in any those dose levels.

MONITORING DATA
water temperature: 19.6-20.1 °C
dissolved oxygen: 7.5-8.6mg/L
              (Saturated concentration at 20°C is 8.8mg/L.)
pH: 7.8-8.2
hardness: 60-80 mg/L as CaCO3

REMARK
This study was the test up to 10 mg/L, due to author's
misunderstanding to the solubility in water. Therefore it was not a real limit test.

Applicant's summary and conclusion

Conclusions:

21 days EC50(and EC0) to parental Daphnia magna : > 10mg/L
21 days NOEC (reproduction) to Daphnia magna : = 3.2mg/L
21 days LOEC (reproduction) to Daphnia magna : = 10mg/L