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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally acceptad scientific standards, well documented and acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report date:
1968

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
yes
Remarks:
0.05 ml of the test substance was instillated in the eye.
Principles of method if other than guideline:
BASF-Test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Dimethyl sulphate
EC Number:
201-058-1
EC Name:
Dimethyl sulphate
Cas Number:
77-78-1
Molecular formula:
C2H6O4S
IUPAC Name:
dimethyl sulfate
Details on test material:
- Name of test material (as cited in study report): dimethyl sulfate
- Physical state: liquid

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Age at study initiation: young adult animals
- Weight at study initiation: 2.26 kg (female; 2.4 kg at the end of the study) and 2.35 kg (male; 2.4 kg at the end of the study)

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 ml
Duration of treatment / exposure:
test substance was not washed out
Observation period (in vivo):
up to 8 days
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): test substance was not washed out

SCORING SYSTEM:
Ocular reactions were recorded 10 min, 1, 3, 24, 48 hours, 5, 7, and 8 days after instillation using a BASF-scoring system, which is convertible into the current OECD Draize scheme.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Remarks:
redness
Basis:
mean
Time point:
other: 24-48-72-hour
Score:
1.7
Max. score:
3
Reversibility:
other: corneal opacity, and strong chemosis with suppuration and staphyloma remained at the end of the observation period
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24-48-72-hour
Score:
2
Max. score:
4
Reversibility:
not reversible
Irritant / corrosive response data:
The test substance is corrosive according to the EU and to GHS
Other effects:
After 1 hour eyes were totally swollen. After 24 hours very strong oedema, strong redness, iritis, bleeding and corneal opacity were reported. Even after 8 days strong redness, corneal opacity, and strong chemosis with suppuration and staphyloma remained.

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information